CaltechAUTHORS
  A Caltech Library Service

Nonenzymatic sequence-specific cleavage of single-stranded DNA to nucleotide resolution. DNA methyl thioether probes

Iverson, Brent L. and Dervan, Peter B. (1987) Nonenzymatic sequence-specific cleavage of single-stranded DNA to nucleotide resolution. DNA methyl thioether probes. Journal of the American Chemical Society, 109 (4). pp. 1241-1243. ISSN 0002-7863. https://resolver.caltech.edu/CaltechAUTHORS:20160511-145020102

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20160511-145020102

Abstract

We report the sequence-specific cleavage of single-stranded DNA to nucleotide resolution using chemically activated oligodeoxynucleotide methyl thioether hybridization probes (DNAMT). 5-[3-[ [3-(Methylthio)propionyl]amino]-trans-1- propenylldeoxyuridine S’-triphosphate (MT-dUTP) was enzymatically incorporated into an oligonucleotide duplex by using the Klenow fragment of DNA polymerase. Activation with CNBr at 25 OC, pH 5.5, followed by treatment with piperidine produced cleavage on the complementary strand at a single guanine (G) residue to the 5’-side of the modified base.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja00238a041DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja00238a041PublisherArticle
ORCID:
AuthorORCID
Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1987 American Chemical Society. Received September 2, 1986. We are grateful to the National Institutes of Health (GM-35724) for support of this research and for a National Research Service Award (T32GM07616) to B.L.I. from the National Institute of General Medical Sciences.
Funders:
Funding AgencyGrant Number
NIHGM-35724
NIH Predoctoral FellowshipT32GM07616
Issue or Number:4
Record Number:CaltechAUTHORS:20160511-145020102
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160511-145020102
Official Citation:Nonenzymatic sequence-specific cleavage of single-stranded DNA to nucleotide resolution. DNA methyl thioether probes Brent L. Iverson and Peter B. Dervan Journal of the American Chemical Society 1987 109 (4), 1241-1243 DOI: 10.1021/ja00238a041
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67013
Collection:CaltechAUTHORS
Deposited By: Victoria Brennan
Deposited On:19 May 2016 20:13
Last Modified:26 Nov 2019 11:15

Repository Staff Only: item control page