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Sequence-specific double-strand cleavage of DNA by bis(EDTA-distamycin•Fe^(II)) and EDTA-bis(distamycin)•Fe^(II)

Schultz, P. G. and Dervan, P. B. (1983) Sequence-specific double-strand cleavage of DNA by bis(EDTA-distamycin•Fe^(II)) and EDTA-bis(distamycin)•Fe^(II). Journal of the American Chemical Society, 105 (26). pp. 7748-7750. ISSN 0002-7863. doi:10.1021/ja00364a049.

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We report the synthesis of two sequence-specific double-strand DNA cleaving molecules, bis(EDTA-distamycin·Fe^(II) (BED·Fe^(II)) and EDTA-bis(distamycin)·Fe^(II) (EBD·Fe^(II)) (Chart 1). These molecules contain two N-methylpyrrole tripeptide units coupled at the amino termini via a flexible tether with EDTA attached to one or both carboxy termini. In the presence of O_2 and dithiothreitol (DTT), nanomolar concentrations of BED·Fe^(II) and EBD·Fe^(II) cleave DNA (25 °C, pH 7.9). BED·Fe^(II) and EBD·Fe^(II) cleave pBR 322 plasmid DNA (4362 base pairs) on opposite strands to afford discrete DNA fragments. High-resolution gel electrophoresis of an end-labeled restriction fragment containing a major binding site reveals cleavage contiguous to an eight base pair sequence 5'-TTTTTATA-3'.

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Dervan, P. B.0000-0001-8852-7306
Additional Information:© 1983 American Chemical Society. Received August 8, 1983. We are grateful to the National Institutes of Health for grant support (GM-27681). Contribution No. 6828, Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125.
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Division of Chemistry and Chemical Engineering Contribution6828
Issue or Number:26
Record Number:CaltechAUTHORS:20160511-152914977
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Official Citation:Sequence-specific double-strand cleavage of DNA by bis(EDTA-distamycin.iron(II)) and EDTA-bis(distamycin).iron(II) P. G. Schultz and P. B. Dervan Journal of the American Chemical Society 1983 105 (26), 7748-7750 DOI: 10.1021/ja00364a049
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67024
Deposited By: Victoria Brennan
Deposited On:19 May 2016 21:13
Last Modified:11 Nov 2021 00:25

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