Schultz, Peter G. and Taylor, John S. and Dervan, Peter B. (1982) Design synthesis of a sequence-specific DNA cleaving molecule. (Distamycin-EDTA)iron(II). Journal of the American Chemical Society, 104 (24). pp. 6861-6863. ISSN 0002-7863. doi:10.1021/ja00388a101. https://resolver.caltech.edu/CaltechAUTHORS:20160511-153737079
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Abstract
The sequence-dependent recognition of nucleic acids by proteins and small molecules is important in the regulation of many biological processes. A large class of these molecules are bifunctional in nature, combining a chemically reactive moiety with a DNA binding unit. One such molecule is the naturally occurring antitumor antibiotic bleomycin, which binds to and cleaves DNA sequence specifically in a reaction that depends on Fe(II) and oxygen. Recently we reported the synthesis of a DNA binding-DNA cleaving molecule, methidiumpropyl-EDTA (MPE). This bifunctional molecule has the DNA intercalator, methidium, tethered to a metal chelator, EDTA. MPE·Fe(II) cleaves double helical DNA in the presence of dithiothreitol (DTT) with efficiencies comparable to those of bleomycin·Fe(Il)/DTT. Unlike bleomycin·Fe(II), MPE·Fe(II) cleaves DNA non-sequence-specifically, consistent with solution studies demonstrating that the intercalator methidium has no overall base composition specificity.
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| Additional Information: | © 1982 American Chemical Society. Received August 16, 1982. We are grateful to the National Institutes of Health for grant support (GM-27681). Contribution No. 6678, Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125. | |||||||||
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| Issue or Number: | 24 | |||||||||
| DOI: | 10.1021/ja00388a101 | |||||||||
| Record Number: | CaltechAUTHORS:20160511-153737079 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160511-153737079 | |||||||||
| Official Citation: | Design synthesis of a sequence-specific DNA cleaving molecule. (Distamycin-EDTA)iron(II) Peter G. Shultz, John S. Taylor, and Peter B. Dervan Journal of the American Chemical Society 1982 104 (24), 6861-6863 DOI: 10.1021/ja00388a101 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 67029 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Victoria Brennan | |||||||||
| Deposited On: | 19 May 2016 21:24 | |||||||||
| Last Modified: | 11 Nov 2021 00:26 |
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