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Identification of two distinct regulatory regions adjacent to the human β-interferon gene

Zinn, Kai and DiMaio, Daniel and Maniatis, Tom (1983) Identification of two distinct regulatory regions adjacent to the human β-interferon gene. Cell, 34 (3). pp. 865-879. ISSN 0092-8674. https://resolver.caltech.edu/CaltechAUTHORS:20160512-164037862

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Abstract

To study the regulation of the human β-interferon (β-IFN) gene by poly(I)-poly(C), we analyzed the expression of deletion mutants of the cloned gene introduced into mouse cells on a new bovine papilloma virus (BPV) vector. In stable cell lines transformed by a BPV-IFN plasmid containing the β-IFN structural gene with 210 bp of DNA to the 5′ side of its mRNA cap site (denoted −210), human β-IFN mRNA is induced ∼400-fold by poly(I)-poly(C), and reproducible levels of expression are observed for independent cell lines. Our studies indicate that there are two distinct regulatory regions adjacent to the gene, located between −77 and −19, and between −210 and −107. The −77 to −19 region is required for constitutive and induced IFN gene expression, and both are drastically reduced by deletion to −73. When sequences between −210 and −107 are deleted, the constitutive level of IFN gene expression is increased 5- to 10-fold, while induced expression is essentially unaffected. Deletion of the −210 to −107 region also alters the kinetics of induction of the gene.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0092-8674(83)90544-5DOIArticle
http://www.sciencedirect.com/science/article/pii/0092867483905445PublisherArticle
ORCID:
AuthorORCID
Zinn, Kai0000-0002-6706-5605
Additional Information:© 1983 MIT. Received July 11, 1983; revised August 8, 1983. We thank Michael Green, Pamela Mellon, Peter Hawley, David Kimelman, and the members of the Maniatis and Melton laboratories for helpful discussions, Tadatsugu Taniguchi for the mouse IFN cDNA clone, and Sophia Stern for help in preparing the manuscript. K. Z. was supported by a National Institutes of Health training grant. D. D. is a Fellow of the Jane Coffin Childs Memorial Fund for Medical Research. This work was supported by grants from the National Institutes of Health and the National Science Foundation. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
NIH Predoctoral FellowshipUNSPECIFIED
Jane Coffin Childs Memorial Fund for Medical ResearchUNSPECIFIED
NSFUNSPECIFIED
Issue or Number:3
Record Number:CaltechAUTHORS:20160512-164037862
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160512-164037862
Official Citation:Kai Zinn, Daniel Dimaio, Tom Maniatis, Identification of two distinct regulatory regions adjacent to the human β-interferon gene, Cell, Volume 34, Issue 3, 1983, Pages 865-879, ISSN 0092-8674, http://dx.doi.org/10.1016/0092-8674(83)90544-5. (http://www.sciencedirect.com/science/article/pii/0092867483905445)
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ID Code:67059
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:13 May 2016 14:57
Last Modified:03 Oct 2019 10:02

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