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Cell lineage-specific programs of expression of multiple actin genes during sea urchin embryogenesis

Cox, Kathleen H. and Angerer, Lynne M. and Lee, James J. and Davidson, Eric H. and Angerer, Robert C. (1986) Cell lineage-specific programs of expression of multiple actin genes during sea urchin embryogenesis. Journal of Molecular Biology, 188 (2). pp. 159-172. ISSN 0022-2836. http://resolver.caltech.edu/CaltechAUTHORS:20160513-092321854

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Abstract

We have determined spatial patterns of expression of individual actin genes in embryos of the sea urchin Strongylocentrotus purpuratus. Radioactively labeled probes specific for each of five cytoplasmic-type (Cy) and the single muscle-type (M) mRNAs were hybridized in situ to sections of fixed embryos. M actin mRNA appears only late in development and is confined to a few cells associated with the coelomic rudiments. The five Cy mRNAs fall into three sets, whose times and sites of expression during development are highly distinctive. Different cell lineages express messages of one or more of these sets, but never all three. Although all Cy actin mRNAs exhibit monophasic accumulation in the RNA of whole embryos during the course of development, such accumulation in many cases results from the summation of both increases and decreases in abundance within individual sets of cells. Within the genomic linkage group CyI-CyIIa-CyIIb, expression of CyI and CyIIb appears to be co-ordinate, and quite distinct from that of CyIIa. CyI and CyIIb are expressed in all lineages at some point in embryogenesis, but confined mainly to oral ectoderm and portions of the gut of the pluteus larva. CyIIa mRNAs are restricted to mesenchyme lineages throughout late gastrula stage, and subsequently accumulate in parts of the gut. The CyIIIa and CyIIIb genes, which form a separate linkage group, are expressed only in aboral ectoderm and its precursors. Furthermore, CyIII messages are the only detectable actin mRNAs in this cell lineage after late blastula stage.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0022-2836(86)90301-3DOIArticle
http://www.sciencedirect.com/science/article/pii/0022283686903013PublisherArticle
Additional Information:© 1986 Elsevier Ltd. Received 17 July 1985. This research was supported by grants from the National Institutes of Health to R.C.A. (GM-25553) and E.H.D. (HD-05753). It was submitted by K.H.C. in partial fulfillment of the requirements for the Ph.D. degree in the Department of Biology, University of Rochester. J.J.L. and K.H.C. were supported by N.I.H. Predoctoral training grants (GM-07616) and GM-07230, respectively). R.C.A. is the recipient of a Career Development Award from the National Institute of Child Health and Human Development.
Funders:
Funding AgencyGrant Number
NIHGM-25553
NIHHD-05753
NIHGM-07616
NIHGM-07230
National Institute of Child Health and Human Development (NICHD)UNSPECIFIED
Record Number:CaltechAUTHORS:20160513-092321854
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160513-092321854
Official Citation:Kathleen H. Cox, Lynne M. Angerer, James J. Lee, Eric H. Davidson, Robert C. Angerer, Cell lineage-specific programs of expression of multiple actin genes during sea urchin embryogenesis, Journal of Molecular Biology, Volume 188, Issue 2, 1986, Pages 159-172, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(86)90301-3. (http://www.sciencedirect.com/science/article/pii/0022283686903013)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67066
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:13 May 2016 18:38
Last Modified:13 May 2016 18:38

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