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Structure and organization of the CyIII actin gene subfamily of the sea urchin, Strongylocentrotus purpuratus

Akhurst, Rosemary J. and Calzone, Frank J. and Lee, James J. and Britten, Roy J. and Davidson, Eric H. (1987) Structure and organization of the CyIII actin gene subfamily of the sea urchin, Strongylocentrotus purpuratus. Journal of Molecular Biology, 194 (2). pp. 193-203. ISSN 0022-2836. https://resolver.caltech.edu/CaltechAUTHORS:20160513-095801104

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Abstract

We describe here the organization of the CyIII subfamily of cytoskeletal actin genes in the sea urchin Strongylocentrotus purpuratus. The functional genes CyIIIa and CyIIIb are linked at a 6 × 10^3-base distance. Gene CyIIIc appears to be a pseudogene that lacks 5′ exons and displays unselected mutational changes. Gene CyIIIa codes for a protein that differs at only nine out of 376 residues from that coded by another cytoskeletal actin gene, CyI. However, five of these nine changes occur within an 11-amino acid region that could represent a functional specialization of the CyIIIa actin protein. The CyIIIa gene possesses three introns, located, respectively, 25 nucleotides upstream from the translation start site, between the codons for amino acids 121 and 122, and within the codon for amino acid 204. These intron positions have also been observed in other cytoskeletal sea urchin actin genes. Comparison of both intron and 3′-terminal sequences shows that the CyIIIa and CyIIIb genes are closely related, while no homology in these untranslated sequences is observed between the CyIII genes and the other cytoskeletal actin genes of the S. purpuratus genome. The CyIII genes probably arose by duplication events at least 40 × 10^6 years ago, prior to radiation of the genus Strongylocentrotus. Consideration of the biological role of the embryo and larval aboral ectoderm cells to which CyIIIa and CyIIIb transcripts are confined suggests that these actins might contribute to cytoskeletal elements that endow the larval body wall with its rigid structure.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0022-2836(87)90368-8DOIArticle
http://www.sciencedirect.com/science/article/pii/0022283687903688PublisherArticle
Additional Information:© 1987 Elsevier Ltd. Received 29 July 1986, and in revised form 22 October 1986. We thank Dr H. Horvath for providing the oligonucleotide primer. This research was supported by NIH grant GM20927. R.A. was supported by an EMBO Fellowship; F.J.C. was supported by a Lieve Fellowship from the American Cancer Society, California Division; and J.J.L. was supported by an NIH training grant (GM07616).
Funders:
Funding AgencyGrant Number
NIHGM-20927
European Molecular Biology Organization (EMBO)UNSPECIFIED
American Cancer Society, California DivisionUNSPECIFIED
NIH Predoctoral FellowshipGM-07616
Issue or Number:2
Record Number:CaltechAUTHORS:20160513-095801104
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160513-095801104
Official Citation:Rosemary J. Akhurst, Frank J. Calzone, James J. Lee, Roy J. Britten, Eric H. Davidson, Structure and organization of the CyIII actin gene subfamily of the sea urchin, Strongylocentrotus purpuratus, Journal of Molecular Biology, Volume 194, Issue 2, 1987, Pages 193-203, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(87)90368-8. (http://www.sciencedirect.com/science/article/pii/0022283687903688)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67068
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:13 May 2016 18:48
Last Modified:03 Oct 2019 10:02

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