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Human β-interferon gene expression is regulated by an inducible enhancer element

Goodbourn, Stephen and Zinn, Kai and Maniatis, Tom (1985) Human β-interferon gene expression is regulated by an inducible enhancer element. Cell, 41 (2). pp. 509-520. ISSN 0092-8674. http://resolver.caltech.edu/CaltechAUTHORS:20160513-122940193

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Abstract

We have localized the regulatory sequence required for viral or poly([)-poly(C) activation of human β-interferon gene expression to a region located between −37 and −77 from the mRNA cap site. This sequence has the characteristics of an inducible enhancer element: it can act upstream or downstream of the β-interferon gene regardless of its orientation, and at distances up to approximately 1 kilobase from its normal location. Moreover, this element can confer inducibility on a heterologous promoter. Further analysis has identified a minimal regulatory element of 14 base pairs within this enhancer. Sequences closely related to this element are present five times within the 5′-flanking regions of both the α- and β-interferon genes. The number of these minimal regulatory elements required for maximal β-interferon gene expression appears to differ in different cell lines.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0092-8674(85)80024-6DOIArticle
http://www.sciencedirect.com/science/article/pii/S0092867485800246PublisherArticle
ORCID:
AuthorORCID
Zinn, Kai0000-0002-6706-5605
Additional Information:© 1985 by MIT. Received March 25, 1985. We thank Tamar Enoch, Julian Banerji, Rick Myers, and Richard Treisman for stimulating discussions and helpful comments on the manuscript; T. Taniguchi for communicating results prior to publication; S. McKnight for providing the tk promoter mutants. S. G. was supported by a Travelling Fellowship from the Medical Research Council of Great Britain, and more recently by fellowship DRG-810 of the Damon Runyon-Walter Winchell Cancer Fund. This work was supported by a grant to T. M. from the National Institutes of Health. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
Medical Research Council (UK)UNSPECIFIED
Damon Runyon-Walter Winchell Cancer FundDRG-810
NIHUNSPECIFIED
Record Number:CaltechAUTHORS:20160513-122940193
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160513-122940193
Official Citation:Stephen Goodbourn, Kai Zinn, Tom Maniatis, Human β-interferon gene expression is regulated by an inducible enhancer element, Cell, Volume 41, Issue 2, 1985, Pages 509-520, ISSN 0092-8674, http://dx.doi.org/10.1016/S0092-8674(85)80024-6. (http://www.sciencedirect.com/science/article/pii/S0092867485800246)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67081
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:13 May 2016 19:39
Last Modified:13 May 2016 19:39

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