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Rh(DIP)_3^(3+): a shape-selective metal complex which targets cruciforms

Kirshenbaum, Mindy R. and Tribolet, Roger and Barton, Jacqueline K. (1988) Rh(DIP)_3^(3+): a shape-selective metal complex which targets cruciforms. Nucleic Acids Research, 16 (16). pp. 7943-7960. ISSN 0305-1048. PMCID PMC338502. http://resolver.caltech.edu/CaltechAUTHORS:20160513-123130441

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Abstract

The coordination complex tris(4,7-diphenylphenanthroline)rhodium(III), Rh(DIP)_3^(3+) binds to and, upon photoactivation, cleaves both DNA strands near the base of a DNA cruciform Sites of photoinduced double-stranded DNA cleavage by the rhodium complex map to regions containing cruciforms on closed circular pBR322, pColE1 and φX174 (replicative form) DNAs. Neither cleavage nor binding by the metal complex, assayed using S1 nuclease, is found on the linear plasmid which lacks the extruded cruciform. High resolution mapping experiments reveal that h(DIP)_3^(3+) cleaves at a specific AT-rich site neighboring the stem of the minor cruciform on pBR322. The primary site of cleavage is found at position 3238 on the 3'-strand and 3250 on the 5-strand and is remarkably specific. The pattern of cleavage, to one side only of the cruciform stem, indicates an asymmetry in the cruciform structure recognized by the complex. These results suggest that Rh(DIP)_3^(3+) may provide a useful reagent to probe cruciform sites. In addition, the high degree of specificity found in targeting the cruciform structure with this simple metal complex underscores the utility of shape-selection for the recognition of specific sites on a DNA strand.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1093/nar/16.16.7943DOIArticle
http://nar.oxfordjournals.org/content/16/16/7943.shortPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC338502/PubMed CentralArticle
ORCID:
AuthorORCID
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© IRL Press Limited, Oxford, England. Received April 28, 1988. Revised and Accepted July 20, 1988. We are grateful to the National Institutes of Health (GM33309), the National Foundation for Cancer Research, and the Swiss National Science Foundation (to R.T.) for their generous support of this research.
Funders:
Funding AgencyGrant Number
NIHGM33309
National Foundation for Cancer ResearchUNSPECIFIED
Swiss National Science Foundation (SNSF)UNSPECIFIED
PubMed Central ID:PMC338502
Record Number:CaltechAUTHORS:20160513-123130441
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160513-123130441
Official Citation:Mindy R. Kirshenbaum, Roger Tribolet, and Jacqueline K. Barton Rh(DIP)33+: a shape-selective metal complex which targets cruciforms Nucl. Acids Res. (1988) 16 (16): 7943-7960 doi:10.1093/nar/16.16.7943
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67082
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:16 May 2016 19:24
Last Modified:16 May 2016 19:24

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