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Latex beads as probes of a neural crest pathway: effects of laminin, collagen, and surface charge on bead translocation

Bronner-Fraser, Marianne (1984) Latex beads as probes of a neural crest pathway: effects of laminin, collagen, and surface charge on bead translocation. Journal of Cell Biology, 98 (6). pp. 1947-1960. ISSN 0021-9525. PMCID PMC2113074. https://resolver.caltech.edu/CaltechAUTHORS:20160516-082837341

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Abstract

In the trunk region of avian embryos, neural crest cells migrate along two pathways: dorsally just under the ectoderm, and ventrally between the neural tube and the somites. Previous work from this laboratory has shown that uncoated latex beads are able to translocate along the ventral neural crest pathway after injection into young embryos; however, beads coated with fibronectin are restricted from the ventral route ( Bronner -Fraser, M.E., 1982, Dev. Biol., 91: 50-63). Here, we extend these observations to determine the effects of other macromolecules on bead distribution. The data show that laminin-coated beads, like fibronectin-coated beads, are restricted from the ventral pathway. In contrast, beads coated with type I collagen translocate ventrally after injection. Because macromolecules have characteristic charge properties, changes in surface charge caused by coating the beads may confound interpretation of the results. Electrostatic effects on bead movement were examined by coating the latex beads with polyamino acids in order to predictably alter the initial surface charge. The surface charge before injection was measured for beads coated with amino acid polymers or with various biologically important macromolecules; the subsequent translocation ability of these beads was then monitored in the embryo. Polylysine-coated beads (positively charged) were restricted from the ventral pathway as were fibronectin and laminin-coated beads, even though fibronectin and laminin beads were both negatively charged. In contrast, polytyrosine -coated beads ( neutrally charged) translocated ventrally as did negatively charged collagen-coated or uncoated beads. The results demonstrate that no correlation exists between the charge properties on the latex bead surface and their subsequent ability to translocate along the ventral pathway. Therefore, an adhesion mechanism independent of surface charge effects must explain the restriction or translocation of latex beads on a neural crest pathway.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1083/jcb.98.6.1947DOIArticle
http://jcb.rupress.org/content/98/6/1947.abstractPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113074/PubMed CentralArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 1984 Rockefeller University Press. The Authors acknowledge that RUP will make the Article freely available to the public on RUP’s website after expiration of the Initial Publication Period, and that RUP intends to submit the Article to PubMed Central in accordance with PubMed Central’s requirements, where the Article will be released to the public after expiration of the Initial Publication Period. After the Initial Publication Period, RUP will grant to the public the non- exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. Received for publication 11 February 1983, and in revised form 13 February 1984. I thank Dr. Scott Fraser for his helpful criticism of the manuscript and Georgia Guillory and Leena Carriere for their technical assistance. In addition, I gratefully acknowledge the aid of Marthe Howard in performing the electrophoresis experiments and Peggy Garrett for helping with the scanning electron microscopy. This work was supported by U.S. Public Health Service grant HD-15527-01 and by Basil O'Connor Starter Research grant 5-312 from the March of Dimes Birth Defects Foundation.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)HD-15527-01
March of Dimes Birth Defects Foundation 5-312
Issue or Number:6
PubMed Central ID:PMC2113074
Record Number:CaltechAUTHORS:20160516-082837341
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160516-082837341
Official Citation:Latex beads as probes of a neural crest pathway: effects of laminin, collagen, and surface charge on bead translocation. M Bronner-Fraser J Cell Biol 1984 98:1947-1960. Published June 1, 1984, doi:10.1083/jcb.98.6.1947
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67097
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:16 May 2016 19:49
Last Modified:03 Oct 2019 10:02

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