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Sequence-selective inhibition of restriction endonucleases by the polyintercalator bis(methidium)spermine

Ikeda, Richard A. and Dervan, Peter B. (1982) Sequence-selective inhibition of restriction endonucleases by the polyintercalator bis(methidium)spermine. Journal of the American Chemical Society, 104 (1). pp. 296-297. ISSN 0002-7863. http://resolver.caltech.edu/CaltechAUTHORS:20160516-091404294

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Abstract

The design of site-specific probes of double helical nucleic acid is complicated by the fact that the critical recognition features of a defined base sequence are still not well understood. We recently reported that the nucleic acid binding affinity of the polyinterialator bis(methidium)spermine (BMSP) was substantially increased over that of the corresponding monomer, ethidium bromide (EB). In addition, from binding studies with nucleic acid homopolymers and copolymers, BMSp was found to have substantially increased sequence specificity. If this sequence- selective binding is found in heterogeneous DNA, BMSp might selectively inhibit DNA-dependent enzymes. To test for selectivity with a noncovalently binding molecule, a "kinetic sampling" assay was devised. We report that the dimer, BMSp, selectively inhibits restriction sites on the pBR322 plasmid, while the monomer, EB, shows no selective action in competition with any of the restriction enzymes utilized.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja00365a058DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja00365a058PublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ja00365a058PublisherSupporting Information
ORCID:
AuthorORCID
Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1982 American Chemical Society. Publication Date: January 1982. We are grateful to the National Institutes of Health (GM27681) for support of this research and to the Fanny and John Hertz Foundtion for a fellowship to R.A.I. We thank M. M. Becker and D. M. Crothers for helpful suggestions.
Funders:
Funding AgencyGrant Number
NIHGM-27681
Fanny and John Hertz FoundtionUNSPECIFIED
Record Number:CaltechAUTHORS:20160516-091404294
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160516-091404294
Official Citation:Sequence-selective inhibition of restriction endonucleases by the polyintercalator bis(methidium)spermine Richard A. Ikeda and Peter B. Dervan Journal of the American Chemical Society 1982 104 (1), 296-297 DOI: 10.1021/ja00365a058
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67099
Collection:CaltechAUTHORS
Deposited By: Victoria Brennan
Deposited On:17 May 2016 16:47
Last Modified:16 Nov 2016 23:22

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