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Sequence-selective inhibition of restriction endonucleases by the polyintercalator bis(methidium)spermine

Ikeda, Richard A. and Dervan, Peter B. (1982) Sequence-selective inhibition of restriction endonucleases by the polyintercalator bis(methidium)spermine. Journal of the American Chemical Society, 104 (1). pp. 296-297. ISSN 0002-7863. doi:10.1021/ja00365a058.

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The design of site-specific probes of double helical nucleic acid is complicated by the fact that the critical recognition features of a defined base sequence are still not well understood. We recently reported that the nucleic acid binding affinity of the polyinterialator bis(methidium)spermine (BMSP) was substantially increased over that of the corresponding monomer, ethidium bromide (EB). In addition, from binding studies with nucleic acid homopolymers and copolymers, BMSp was found to have substantially increased sequence specificity. If this sequence- selective binding is found in heterogeneous DNA, BMSp might selectively inhibit DNA-dependent enzymes. To test for selectivity with a noncovalently binding molecule, a "kinetic sampling" assay was devised. We report that the dimer, BMSp, selectively inhibits restriction sites on the pBR322 plasmid, while the monomer, EB, shows no selective action in competition with any of the restriction enzymes utilized.

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Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1982 American Chemical Society. Publication Date: January 1982. We are grateful to the National Institutes of Health (GM27681) for support of this research and to the Fanny and John Hertz Foundtion for a fellowship to R.A.I. We thank M. M. Becker and D. M. Crothers for helpful suggestions.
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Fanny and John Hertz FoundtionUNSPECIFIED
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Official Citation:Sequence-selective inhibition of restriction endonucleases by the polyintercalator bis(methidium)spermine Richard A. Ikeda and Peter B. Dervan Journal of the American Chemical Society 1982 104 (1), 296-297 DOI: 10.1021/ja00365a058
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67099
Deposited By: Victoria Brennan
Deposited On:17 May 2016 16:47
Last Modified:11 Nov 2021 00:27

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