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Hexane Dissolved in Dioleoyllecithin Bilayers Has a Partial Molar Volume of Approximately Zero

King, Glen I. and Jacobs, Russell E. and White, Stephen H. (1985) Hexane Dissolved in Dioleoyllecithin Bilayers Has a Partial Molar Volume of Approximately Zero. Biochemistry, 24 (17). pp. 4637-4645. ISSN 0006-2960. http://resolver.caltech.edu/CaltechAUTHORS:20160516-132218461

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Abstract

Neutron diffraction has been used to measure the amount and distribution of hexane incorporated from the vapor phase into oriented dioleoylphosphatidylcholine bilayers at 66% relative humidity. We reported earlier that hexane at low concentrations is located largely in a zone 10 Å wide at the center of the bilayer [White, S. H., King, G. I., & Cain, J.E. (1981) Nature (London) 290, 161-163]. Extending these studies to high hexane concentrations, we find no readily apparent change in the volume of the hydrocarbon region of the bilayer even though more than one hexane molecule per lipid enters the region. The hexane partial molar volume in the bilayer hydrocarbon region is thus approximately zero. Within our statistical confidence limits, the partial molar volume is certainly no greater than one-third the molecular volume of the hexane. Further, analysis of the data suggests that the mass density of the bilayer is considerably less than 1 in the absence of hexane. These findings are in conflict with the assumptions usually made about lipid bilayers and their interaction with nonpolar hydrophobic molecules. In the course of these experiments, we found that standard methods of interpreting diffraction results were not suitable for our purposes. We thus developed several new methods which are summarized in the text and two appendixes. One of these methods allows us to define with precision the width of the hydrocarbon core of the bilayer. The other provides a means of calculating the effects of changes in the absolute scaling of the bilayers with changes in composition without placing the structures on an absolute scattering length density scale.


Item Type:Article
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http://dx.doi.org/10.1021/bi00338a024DOIArticle
http://pubs.acs.org/doi/abs/10.1021/bi00338a024PublisherArticle
ORCID:
AuthorORCID
Jacobs, Russell E.0000-0002-1382-8486
Additional Information:© 1985 American Chemical Society. Received July 26, 1984; Revised Manuscript Received November 30, 1984. Parts of this research were carried out at the Brookhaven National Laboratory under the auspices of the U.S. Department of Energy with the additional support of the National Science Foundation. This research was supported by grants from the National Institutes of Health and National Science Foundation. Parts of this work were presented in preliminary form at the Brookhaven Symposium in Biology, May 31-June 4, 1982.
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Record Number:CaltechAUTHORS:20160516-132218461
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160516-132218461
Official Citation:Hexane dissolved in dioleoyllecithin bilayers has a partial molar volume of approximately zero Glen I. King, Russell E. Jacobs, and Stephen H. White Biochemistry 1985 24 (17), 4637-4645 DOI: 10.1021/bi00338a024
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67134
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:16 May 2016 21:56
Last Modified:16 May 2016 21:56

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