Bell, John R. and Rice, Charles M. and Hunkapiller, Michael W. and Strauss, James H. (1982) The N-Terminus of PE2 in Sindbis Virus-Infected Cells. Virology, 119 (2). pp. 255-267. ISSN 0042-6822. doi:10.1016/0042-6822(82)90086-1. https://resolver.caltech.edu/CaltechAUTHORS:20160516-151803038
Full text is not posted in this repository. Consult Related URLs below.
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20160516-151803038
Abstract
One of the two envelope glycoproteins of Sindbis virus, E2, and its intracellular precursor PE2 were obtained in chemically pure form from Sindbis infected cells by immunoabsorption with antibody against E2. The N-terminus of PE2, which is probably the signal sequence of this protein, was examined and two N-termini were found. One, the primary product of translation, was sequenced by Edman degradation and the other was found to be blocked and produced by N-acetylation of the first. It was also found that an asparagine residue at position 14 of PE2 is glycosylated. The putative signal sequence is not cleaved by signal peptidase either under normal conditions or when glycosylation is prevented with tunicamycin.
| Item Type: | Article | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Related URLs: |
| ||||||||||
| Additional Information: | © 1982 by Academic Press, Inc. Received August 4, 1981; accepted February 11, 1982. We wish to thank L. E. Hood for this advice and assistance and E. Lenches for preparing the chick embryo fibroblasts. This investigation was supported by Grants GM06965 and AI10793 from the National Institutes of Health and by Grant PCM80-22830 from the National Science Foundation. J.R.B. was supported in part by training Grant GM00086 from the National Institutes of Health. | ||||||||||
| Funders: |
| ||||||||||
| Issue or Number: | 2 | ||||||||||
| DOI: | 10.1016/0042-6822(82)90086-1 | ||||||||||
| Record Number: | CaltechAUTHORS:20160516-151803038 | ||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160516-151803038 | ||||||||||
| Official Citation: | John R. Bell, Charles M. Rice, Michael W. Hunkapiller, James H. Strauss, The N-terminus of PE2 in Sindbis virus-infected cells, Virology, Volume 119, Issue 2, 1982, Pages 255-267, ISSN 0042-6822, http://dx.doi.org/10.1016/0042-6822(82)90086-1. (http://www.sciencedirect.com/science/article/pii/0042682282900861) | ||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||
| ID Code: | 67137 | ||||||||||
| Collection: | CaltechAUTHORS | ||||||||||
| Deposited By: | Donna Wrublewski | ||||||||||
| Deposited On: | 17 May 2016 15:01 | ||||||||||
| Last Modified: | 11 Nov 2021 00:27 |
Repository Staff Only: item control page
