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Rat Serum Contains a Developmentally Regulated Cholinergic Inducing Activity

Wolinsky, Eve J. and Patterson, Paul H. (1985) Rat Serum Contains a Developmentally Regulated Cholinergic Inducing Activity. Journal of Neuroscience, 5 (6). pp. 1509-1512. ISSN 0270-6474. PMCID PMC6565265. doi:10.1523/jneurosci.05-06-01509.1985.

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Sympathetic neurons cultured in defined medium do not develop the ability to produce acetylcholine, as do neurons grown with serum supplementation (lacovitti, L., M. I. Johnson, T. H. Joh, and R. P. Bunge (1982) Neuroscience 7:2225–2239; Wolinsky, E. J., S. C. Landis, and P. H. Patterson (1985) J. Neurosci. 5: 1497–1508). The implication that rat serum contains cholinergic inducing activity is further explored here. Dependence of cholinergic induction on serum concentration is demonstrated, and the activity is shown to reside in a macromolecular fraction. Very little cholinergic inducing activity is present in serum obtained from animals younger than 9 postnatal days. This age dependence correlates with the time of transition from noradrenergic to cholinergic transmitter status by the sympathetic innervation of the rat sweat gland in vivo (Landis, S. C., and D. Keefe (1983) Dev. Biol. 98: 349–372).

Item Type:Article
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Additional Information:© 1985 Society for Neuroscience. For the first six months after publication SfN’s license will be exclusive. Beginning six months after publication the Work will be made freely available to the public on SfN’s website to copy, distribute, or display under a Creative Commons Attribution 4.0 International (CC BY 4.0) license ( Received July 2, 1984; Revised October 5, 1984; Accepted October 8, 1984. This work was supported by grants from the National Institute of Neurological and Communicative Disorders and Stroke. E. J. W. was a predoctoral trainee of the National Institute of General Medical Sciences. P. H. P. was a Rita Allen Foundation Fellow and a McKnight Foundation Neuroscience Development Awardee. Support was also provided by grants from the National Institute of Neurological and Communicative Disorders and Stroke to P. H. P. We would like to thank Doreen McDowell for assistance with the cell culturing. We also thank Ors. S. C. Landis and T. M. Jessell for helpful discussions of the manuscript.
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National Institute of Neurological and Communicative Disorders and Stroke (NINCDS)UNSPECIFIED
National Institute of General Medical SciencesUNSPECIFIED
Rita Allen FoundationUNSPECIFIED
McKnight FoundationUNSPECIFIED
Issue or Number:6
PubMed Central ID:PMC6565265
Record Number:CaltechAUTHORS:20160517-082439647
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Official Citation:Rat serum contains a developmentally regulated cholinergic inducing activity EJ Wolinsky and PH Patterson The Journal of Neuroscience, 1 June 1985, 5(6):1509-1512
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67150
Deposited By: Ruth Sustaita
Deposited On:17 May 2016 16:12
Last Modified:11 Nov 2021 00:28

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