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Insulin Promotes Electrical Coupling between Cultured Sympathetic Neurons

Wolinsky, Eve J. and Patterson, Paul H. and Willard, Alan L. (1985) Insulin Promotes Electrical Coupling between Cultured Sympathetic Neurons. Journal of Neuroscience, 5 (7). pp. 1675-1679. ISSN 0270-6474. https://resolver.caltech.edu/CaltechAUTHORS:20160517-083209900

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Abstract

Placing neurons in tissue culture is one way to study how environmental factors affect their differentiation. Replacement of serum- supplementation of the culture medium with defined ingredients extends the experimenter's control of the culture environment; however it also introduces additional potential influences. In this report, we confirm the observation of Higgins and Burton (Higgins, D., and H. Burton (1982) Neuroscience 7:2241–2253) of increased frequency of electrical coupling in serum-free compared to serum-supplemented cultures of rat sympathetic neurons. In addition, experiments were performed to determine whether this effect results from the removal of serum or from the addition of the defined medium components to the culture environment. The results of testing individual ingredients of the defined medium recipe adapted for use on sympathetic neurons (Bottenstein, J.E., and G. H. Sato (1979) Proc. Natl. Acad. Sci. U. S. A. 76:514–517) show that insulin is capable of inducing electrical coupling in serum-free cultures. Thus, the formation of electrical synapses by sympathetic neurons can be hormonally regulated.


Item Type:Article
Related URLs:
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http://www.jneurosci.org/content/5/7/1675.shortPublisherArticle
Additional Information:© 1985 Society for Neuroscience. For the first six months after publication SfN’s license will be exclusive. Beginning six months after publication the Work will be made freely available to the public on SfN’s website to copy, distribute, or display under a Creative Commons Attribution 4.0 International (CC BY 4.0) license (https://creativecommons.org/licenses/by/4.0/). Received July 2, 1984; Revised October 5, 1984; Accepted October 8, 1984. This work was supported by grants from the National Institute of Neurological and Communicative Disorders and Stroke (NINCDS). E. J. W. was a predoctoral trainee of the National Institute of General Medical Sciences. P. H. P. was a Rita Allen Foundation Fellow and a McKnight Foundation Neuroscience Development Awardee. A. L. W. was a postdoctoral fellow of the American Heart Association, Massachusetts Affiliate, and is an Alfred P. Sloan Research Fellow. Support was also provided by grants from NINCDS to P. H. P. We would like to thank Doreen McDowell for assistance with the cell culturing. We also thank Ors. S. C. Landis and T. M. Jessell for helpful discussion of the manuscript and Dr. E. J. Furshpan for help with initial experiments.
Funders:
Funding AgencyGrant Number
National Institute of Neurological and Communicative Disorders and Stroke (NINCDS)UNSPECIFIED
NIH Predoctoral FellowshipUNSPECIFIED
Rita Allen FoundationUNSPECIFIED
McKnight FoundationUNSPECIFIED
American Heart Association, Massachusetts AffiliateUNSPECIFIED
Alfred P. Sloan FoundationUNSPECIFIED
Issue or Number:7
Record Number:CaltechAUTHORS:20160517-083209900
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160517-083209900
Official Citation:Insulin promotes electrical coupling between cultured sympathetic neurons EJ Wolinsky, PH Patterson, and AL Willard The Journal of Neuroscience, 1 July 1985, 5(7):1675-1679
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67151
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:17 May 2016 17:14
Last Modified:03 Oct 2019 10:03

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