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The Influence of Neural Tube-derived Factors on Differentiation of Neural Crest Cells In Vitro.I. Histochemical Study on the Appearance of Adrenergic Cells

Howard, Marthe J. and Bronner-Fraser, Marianne (1985) The Influence of Neural Tube-derived Factors on Differentiation of Neural Crest Cells In Vitro.I. Histochemical Study on the Appearance of Adrenergic Cells. Journal of Neuroscience, 5 (12). pp. 3302-3309. ISSN 0270-6474. http://resolver.caltech.edu/CaltechAUTHORS:20160517-104614514

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Abstract

The neural crest gives rise to numerous derivatives including adrenergic and cholinergic neurons, supportive cells of the nervous system, and melanocytes. In tissue culture, neural crest cells explanted from both cranial and trunk regions were found to differentiate into adrenergic neuroblasts or into pigmented cells when grown in medium containing 10% chick embryo extract. When the embryo extract concentration was lowered to 2%, no catecholamine-containing cells (as assayed by formaldehyde-induced fluorescence) were detected, although pigment cells were observed. These results suggest the presence of a factor(s) in embryo extract that promotes or supports adrenergic differentiation. To examine the possible tissue sources of this factor(s), neural tube, notochord, or somite cells were used to condition medium containing 2% embryo extract. When neural crest cells were grown in medium conditioned by neural tube cells, adrenergic neuroblasts were observed in all cultures. However, somite- and notochord conditioned medium did not support adrenergic differentiation. In addition, medium supplemented with extracts derived from central nervous system components did support adrenergic expression, whereas medium supplemented with embryo extract from which the neural tissue was removed did not. Direct contact with neural tube cell ghost membranes was unable to substitute for high embryo extract concentrations or for neural tube-conditioned medium. These results suggest that the neural tube makes a diffusible factor(s) that will support adrenergic differentiation of neural crest cells.


Item Type:Article
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http://www.jneurosci.org/content/5/12/3302.shortPublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 1985 Society for Neuroscience. For the first six months after publication SfN’s license will be exclusive. Beginning six months after publication the Work will be made freely available to the public on SfN’s website to copy, distribute, or display under a Creative Commons Attribution 4.0 International (CC BY 4.0) license (https://creativecommons.org/licenses/by/4.0/). Received February 27, 1985; Revised April 22, 1985; Accepted April 22, 1985. We thank Drs. Scott Fraser and James Coulombe for helpful comments on the manuscript, and Georgia Guillory for her excellent technical assistance. This work was partially supported by United States Public Health Service Grant 15527-01 and a Basic Research grant for the March of Dimes Birth Defects Foundation.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)15527-01
March of Dimes Birth Defects FoundationUNSPECIFIED
Record Number:CaltechAUTHORS:20160517-104614514
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160517-104614514
Official Citation:The influence of neural tube-derived factors on differentiation of neural crest cells in vitro. I. Histochemical study on the appearance of adrenergic cells MJ Howard and M Bronner-Fraser The Journal of Neuroscience, 1 December 1985, 5(12):3302-3309
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67157
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:17 May 2016 19:25
Last Modified:17 May 2016 19:25

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