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Mitochondrial DNA Sequences in the Nuclear Genome of Strongylocentrotus purpuratus

Jacobs, Howard T. and Posakony, James W. and Grula, John W. and Roberts, John W. and Xin, Ji-Hou and Britten, Roy J. and Davidson, Eric H. (1983) Mitochondrial DNA Sequences in the Nuclear Genome of Strongylocentrotus purpuratus. Journal of Molecular Biology, 165 (4). pp. 609-632. ISSN 0022-2836. https://resolver.caltech.edu/CaltechAUTHORS:20160518-090554349

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Abstract

Two sea urchin embryo complementary DNA clones representing mitochondrial 16 S ribosomal RNA and cytochrome oxidase subunit I messenger RNA have been characterized. The cloned cDNAs are colinear with sea urchin mitochondrial DNA, and their identification is based on cross-hybridization with known restriction fragments of human mitochondrial DNA, and on nucleotide sequence determinations. The mitochondrial cDNA clones also displayed an unexpected reaction with specific genomic DNA sequences in gel blot hybridizations. Genomic phage λ recombinants containing sequences hybridizing with the mitochondrial clones were isolated and the arrangement of these sequences was determined. The genomic region studied contains a sequence homologous with the 3′ end of the mitochondrial 16 S rRNA gene, flanked on one side by what is possibly a complete copy of the cytochrome oxidase subunit I gene, and on the other by a duplication of a fragment of this gene. The nucleotide sequence divergence between the mitochondrial and nuclear homologues of the cytochrome oxidase subunit I gene varies for different regions of the gene, from about 13% to 25%, while there is about 8% sequence divergence between nuclear and mitochondrial versions of the 3′ 16 S rRNA sequence. The structure of the genomic mitochondrial sequence homologues indicates that during sea urchin evolution there occurred a germ-line transposition of a fragment of the mitochondrial genome into the nuclear DNA, followed by rearrangements and single nucleotide substitutions.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0022-2836(83)80270-8DOIArticle
http://www.sciencedirect.com/science/article/pii/S0022283683802708PublisherArticle
Additional Information:© 1983 Academic Press Inc. (London) Ltd. Received 19 October 1982, and in revised form 10 December 1982. Edited by W. Franke. We acknowledge the helpful comments of Dr G. Attardi and the technical assistance of Mr Tracy Furutani, who was supported by a SURF fellowship. This research was supported by a National Institutes of Health grant GM-20927. One of the authors (H.T.J.) was supported by a NATO-SRC fellowship, one (J.W.P.) by a National Institutes of Health Service award (GM-07616), and one (J.W.G.) by a National Institutes of Health postdoctoral fellowship (GM-06865).
Funders:
Funding AgencyGrant Number
Caltech Summer Undergraduate Research Fellowship (SURF)UNSPECIFIED
NIHGM-20927
North Atlantic Treaty Organization (NATO)UNSPECIFIED
NIH Predoctoral FellowshipGM-07616
NIH Postdoctoral FellowshipGM-06865
Issue or Number:4
Record Number:CaltechAUTHORS:20160518-090554349
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160518-090554349
Official Citation:Howard T. Jacobs, James W. Posakony, John W. Grula, John W. Roberts, Ji-Hou Xin, Roy J. Britten, Eric H. Davidson, Mitochondrial DNA sequences in the nuclear genome of Strongylocentrotus pupuratus, Journal of Molecular Biology, Volume 165, Issue 4, 1983, Pages 609-632, ISSN 0022-2836, http://dx.doi.org/10.1016/S0022-2836(83)80270-8. (http://www.sciencedirect.com/science/article/pii/S0022283683802708)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67169
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:18 May 2016 18:25
Last Modified:03 Oct 2019 10:03

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