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Design of synthetic sequence specific DNA binding molecules

Dervan, Peter B. (1987) Design of synthetic sequence specific DNA binding molecules. In: Working Group on Molecular Mechanisms of Carcinogenic and Antitumor Activity, October 21-25, 1986. Pontificiae Academiae Scientiarum scripta varia. No.70. Pontificiae Academiae Scientiarum , Vatican City, pp. 365-384. ISBN 9788877610232. https://resolver.caltech.edu/CaltechAUTHORS:20160519-124109662

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Abstract

The design of sequence specific DNA binding molecules has advanced in recent years due, in part, to analytical techniques such as footprinting and affinity cleaving which allow rapid and precise analysis of hundreds of potential DNA binding sites on sequencing gels (Dervan, 1986 ). Nona-N-methylpyrrolecarboxamide, a synthetic analog of the natural product distamycin, binds 11 contiguous base pairs of A,T rich DNA in the minor groove. A synthetic polypeptide, 52 amino acid residues in length and derived from a recombinase protein, binds uniquely 12 base pairs of DNA, most likely in the major and minor groove. The construction of synthetic molecules that bind in the minor and major groove of DNA with incrementally increasing sequence specificity is the first step toward defining a set of rules for the three-dimensional readout of double helical DNA. This may lead to new research tools for use in cancer research, diagnosis of disease states at the level of DNA (oncogenes), and novel chemotherapeutic strategies such as artificial repressors for inactivation of these genes.


Item Type:Book Section
ORCID:
AuthorORCID
Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1987 Pontificiae Academiae Scientiarum. We are very grateful for the generous support of the National Institutes of Health, the American Cancer Society, the National Foundation for Cancer Research, the Burroughs-Wellcome Company, and the Smith Kline Beckman Corporation.
Funders:
Funding AgencyGrant Number
NIHUNSPECIFIED
American Cancer SocietyUNSPECIFIED
National Foundation for Cancer ResearchUNSPECIFIED
Burroughs-WellcomeUNSPECIFIED
Smith Kline BeckmanUNSPECIFIED
Series Name:Pontificiae Academiae Scientiarum scripta varia
Issue or Number:70
Record Number:CaltechAUTHORS:20160519-124109662
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160519-124109662
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67182
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:20 May 2016 20:17
Last Modified:26 Nov 2019 11:15

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