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Chemical methods for the site-specific cleavage of genomic DNA

Dervan, Peter B. (1990) Chemical methods for the site-specific cleavage of genomic DNA. In: Structure and Methods. Vol.1. Adenine Press , pp. 37-49. ISBN 0-940030-29-2. https://resolver.caltech.edu/CaltechAUTHORS:20160519-124144205

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Abstract

Pyrimidine oligodeoxyribonucleotides bind duplex DNA at homopurine sites to form a triple helix structure. The pyrimidine oligodeoxyribonucleotide is oriented in the major groove of DNA parallel to the Watson-Crick purine strand. Specificity is due to Hoogsteen hydrogen bonds wherein T recognizes A-T base pairs (T•AT triplet) and protonated C recognizes GC base pairs (C + GC triplet). Oligonucleotides 18 bases in length, equipped with a DNA cleaving function EDTA•Fe at the 5' end. cause sequence specific double strand breaks at one site in the 48.5 kbp bacteriophage λ genome. Due to the length of the recognition site, in a formal sense. this is 10^6 times more sequence-specific that restriction enzymes. The triple helix motif can be extended from homopurine target sequences to mixed sequences. Oligonucleotide directed triple helix formation could be useful for mapping genomic DNA.


Item Type:Book Section
ORCID:
AuthorORCID
Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1990 Adenine Press. We thank the National Institutes of Health (GM-35724), the Office of Naval Research, the Parsons Foundation, Burroughs Wellcome, and Merck for generous financial support.
Funders:
Funding AgencyGrant Number
NIHGM-35724
Office of Naval Research (ONR)UNSPECIFIED
Ralph M. Parsons FoundationUNSPECIFIED
Burroughs WellcomeUNSPECIFIED
MerckUNSPECIFIED
Record Number:CaltechAUTHORS:20160519-124144205
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160519-124144205
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67185
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:20 May 2016 15:17
Last Modified:26 Nov 2019 11:15

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