Dervan, Peter B. (1990) Chemical methods for the site-specific cleavage of genomic DNA. In: Structure and Methods. Vol.1. Adenine Press , pp. 37-49. ISBN 0-940030-29-2. https://resolver.caltech.edu/CaltechAUTHORS:20160519-124144205
Full text is not posted in this repository.
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20160519-124144205
Abstract
Pyrimidine oligodeoxyribonucleotides bind duplex DNA at homopurine sites to form a triple helix structure. The pyrimidine oligodeoxyribonucleotide is oriented in the major groove of DNA parallel to the Watson-Crick purine strand. Specificity is due to Hoogsteen hydrogen bonds wherein T recognizes A-T base pairs (T•AT triplet) and protonated C recognizes GC base pairs (C + GC triplet). Oligonucleotides 18 bases in length, equipped with a DNA cleaving function EDTA•Fe at the 5' end. cause sequence specific double strand breaks at one site in the 48.5 kbp bacteriophage λ genome. Due to the length of the recognition site, in a formal sense. this is 10^6 times more sequence-specific that restriction enzymes. The triple helix motif can be extended from homopurine target sequences to mixed sequences. Oligonucleotide directed triple helix formation could be useful for mapping genomic DNA.
| Item Type: | Book Section | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ORCID: |
| ||||||||||||
| Additional Information: | © 1990 Adenine Press. We thank the National Institutes of Health (GM-35724), the Office of Naval Research, the Parsons Foundation, Burroughs Wellcome, and Merck for generous financial support. | ||||||||||||
| Funders: |
| ||||||||||||
| Record Number: | CaltechAUTHORS:20160519-124144205 | ||||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160519-124144205 | ||||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||
| ID Code: | 67185 | ||||||||||||
| Collection: | CaltechAUTHORS | ||||||||||||
| Deposited By: | George Porter | ||||||||||||
| Deposited On: | 20 May 2016 15:17 | ||||||||||||
| Last Modified: | 26 Nov 2019 11:15 |
Repository Staff Only: item control page
