Synthetic sequence specific DNA binding molecules

Abstract

The design of sequence specific DNA-binding molecules has advanced in recent years due, in part, to the development of analytical techniques such as footprinting and affinity cleaving which allow rapid and precise analysis of hundreds of of potential DNA binding sites. The construction of synthetic molecules that bind in the minor and major groove of DNA with incrementally increasing sequence specificity is simply the first step toward developing a set of rules for the three-dimensional readout of double helical DNA.