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15N NMR Studies of Nitrogen Metabolism in Intact Mycelia of Neurospora crassa

Legerton, Timothy L. and Kanamori, Keiko and Weiss, Richard L. and Roberts, John D. (1981) 15N NMR Studies of Nitrogen Metabolism in Intact Mycelia of Neurospora crassa. Proceedings of the National Academy of Sciences of the United States of America, 78 (3). pp. 1495-1498. ISSN 0027-8424.

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Suspensions of intact mycelia of Neurospora crassa grown in medium containing 15NH4Cl have been found to give well-resolved 15N nuclear magnetic resonance spectra for metabolites that play crucial roles in intermediary nitrogen metabolism. These include the amide nitrogen of glutamine, the alpha-amino nitrogens of glutamate and other amino acids, the guanidino nitrogens of arginine, the ureido nitrogen of citrulline, the side-chain nitrogens of ornithine or lysine, or both, and uridine diphosphates. The turnover time of glutamine in vivo was estimated to be less than 1 hr by using nuclear magnetic resonance spectroscopy in conjunction with tracer methodologies. Applications of these techniques to the study of nitrogen metabolism are discussed.

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Additional Information:Copyright © 1981 by the National Academy of Sciences Contributed by John D. Roberts, November 24, 1980 This work was supported in part by Grant PCM78-19030 from the National Science Foundation (R.L.W.), a Biomedical Research Support Grant to the University of California, Los Angeles; by U.S. Public Health Service Research Grant GM-11072 from the Division of General Medical Sciences; and by the National Science Foundation at the California Institute of Technology. T.L.L. was supported by U.S. Public Health Service National Research Award 07104. This is contribution no. 6349 from the Gates and Crellin Laboratories of Chemistry.
Funding AgencyGrant Number
NSFPCM 78-19030
U.S. Public Health Service (USPHS)GM-11072
U.S. Public Health Service (USPHS)07104
Subject Keywords:amino acid biosynthesis; metabolic turnover
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Caltech Gates and Crellin Laboratories of Chemistry6349
Issue or Number:3
Record Number:CaltechAUTHORS:LEGpnas81
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:6719
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Deposited On:19 Dec 2006
Last Modified:02 Oct 2019 23:35

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