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A regulatory domain that directs lineage-specific expression of a skeletal matrix protein gene in the sea urchin embryo

Sucov, Henry M. and Hough-Evans, Barbara R. and Franks, Roberta R. and Britten, Roy J. and Davidson, Eric H. (1988) A regulatory domain that directs lineage-specific expression of a skeletal matrix protein gene in the sea urchin embryo. Genes and Development, 2 (10). pp. 1238-1250. ISSN 0890-9369. http://resolver.caltech.edu/CaltechAUTHORS:20160520-104219073

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Abstract

DNA sequences derived from the 5' region of a gene coding for the 50-kD skeletal matrix protein (SM50) of sea urchin embryo spicules were linked to the CAT reporter gene and injected into unfertilized eggs. CAT mRNA and enzyme were synthesized from these fusion constructs in embryos derived from these eggs, and in situ hybridization with a CAT antisense RNA probe demonstrated that expression is confined to skeletogenic mesenchyme cells. A mean of 5.5 of the 32-blastula-stage skeletogenic mesenchyme cells displayed CAT mRNA (range 1-15), a result consistent with earlier measurements indicating that incorporation of the exogenous injected DNA probably occurs in a single blastomere during early cleavage. In vitro mutagenesis and deletion experiments showed that CAT enzyme activity in the transgenic embryos is enhanced 34-fold by decreasing the number of SM50 amino acids at the amino-terminus of the fusion protein from 43 to 4. cis-regulatory sequences that are sufficient to promote lineage-specific spatial expression in the embryo are located between -440 and +120 with respect to the transcriptional initiation site.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1101/gad.2.10.123DOIArticle
http://genesdev.cshlp.org/content/2/10/1238.abstractPublisherArticle
Additional Information:© 1988 Cold Spring Harbor Laboratory Press. The Authors acknowledge that six months after the full-issue publication date, the Article will be distributed under a Creative Commons CC-BY-NC License (Attribution-NonCommercial 4.0 International License, http://creativecommons.org/licenses/by-nc/4.0/). Received July 7, 1988; revised version accepted August 18, 1988. This research was supported by National Institutes of Health Grant HD-05753. H.M.S. was supported by NIH Training Grant GM-07616.
Funders:
Funding AgencyGrant Number
NIHHD-05753
NIHGM-07616
Subject Keywords:Transgenic embryo; spicule; cis-regnlatory sequences
Record Number:CaltechAUTHORS:20160520-104219073
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160520-104219073
Official Citation:A regulatory domain that directs lineage-specific expression of a skeletal matrix protein gene in the sea urchin embryo. H M Sucov, B R Hough-Evans, R R Franks, R J Britten, and E H Davidson Genes Dev. October 1988 2: 1238-1250; doi:10.1101/gad.2.10.1238
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67202
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:20 May 2016 18:13
Last Modified:20 May 2016 18:13

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