Sucov, Henry M. and Hough-Evans, Barbara R. and Franks, Roberta R. and Britten, Roy J. and Davidson, Eric H. (1988) A regulatory domain that directs lineage-specific expression of a skeletal matrix protein gene in the sea urchin embryo. Genes and Development, 2 (10). pp. 1238-1250. ISSN 0890-9369. doi:10.1101/gad.2.10.123. https://resolver.caltech.edu/CaltechAUTHORS:20160520-104219073
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Abstract
DNA sequences derived from the 5' region of a gene coding for the 50-kD skeletal matrix protein (SM50) of sea urchin embryo spicules were linked to the CAT reporter gene and injected into unfertilized eggs. CAT mRNA and enzyme were synthesized from these fusion constructs in embryos derived from these eggs, and in situ hybridization with a CAT antisense RNA probe demonstrated that expression is confined to skeletogenic mesenchyme cells. A mean of 5.5 of the 32-blastula-stage skeletogenic mesenchyme cells displayed CAT mRNA (range 1-15), a result consistent with earlier measurements indicating that incorporation of the exogenous injected DNA probably occurs in a single blastomere during early cleavage. In vitro mutagenesis and deletion experiments showed that CAT enzyme activity in the transgenic embryos is enhanced 34-fold by decreasing the number of SM50 amino acids at the amino-terminus of the fusion protein from 43 to 4. cis-regulatory sequences that are sufficient to promote lineage-specific spatial expression in the embryo are located between -440 and +120 with respect to the transcriptional initiation site.
Item Type: | Article | |||||||||
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Additional Information: | © 1988 Cold Spring Harbor Laboratory Press. The Authors acknowledge that six months after the full-issue publication date, the Article will be distributed under a Creative Commons CC-BY-NC License (Attribution-NonCommercial 4.0 International License, http://creativecommons.org/licenses/by-nc/4.0/). Received July 7, 1988; revised version accepted August 18, 1988. This research was supported by National Institutes of Health Grant HD-05753. H.M.S. was supported by NIH Training Grant GM-07616. | |||||||||
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Subject Keywords: | Transgenic embryo; spicule; cis-regnlatory sequences | |||||||||
Issue or Number: | 10 | |||||||||
DOI: | 10.1101/gad.2.10.123 | |||||||||
Record Number: | CaltechAUTHORS:20160520-104219073 | |||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160520-104219073 | |||||||||
Official Citation: | A regulatory domain that directs lineage-specific expression of a skeletal matrix protein gene in the sea urchin embryo. H M Sucov, B R Hough-Evans, R R Franks, R J Britten, and E H Davidson Genes Dev. October 1988 2: 1238-1250; doi:10.1101/gad.2.10.1238 | |||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
ID Code: | 67202 | |||||||||
Collection: | CaltechAUTHORS | |||||||||
Deposited By: | Ruth Sustaita | |||||||||
Deposited On: | 20 May 2016 18:13 | |||||||||
Last Modified: | 11 Nov 2021 00:28 |
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