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Alteration of the retinotectal map in Xenopus by antibodies to neural cell adhesion molecules

Fraser, Scott E. and Murray , Ben A. and Chuong, Cheng Ming and Edelman, Gerald M. (1984) Alteration of the retinotectal map in Xenopus by antibodies to neural cell adhesion molecules. Proceedings of the National Academy of Sciences of the United States of America, 81 (13). pp. 4222-4226. ISSN 0027-8424. PMCID PMC345401. https://resolver.caltech.edu/CaltechAUTHORS:20160520-111325950

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Abstract

The neural cell adhesion molecule (N-CAM) mediates neuron-neuron adhesion, is ubiquitous in the nervous system of developing and mature vertebrates, and undergoes major alterations in both amount and distribution during development. Perturbation of homophillic (N-CAM to N-CAM) building by univalent fragments of specific anti-N-CAM antibodies has previously been found to alter neural patterns in vitro. To show that significant alterations can also occur in vivo, antibodies lo Xenopus N-CAM were embedded in agarose microcylinders and implanted in the tecta of juvenile Xenopus laevis frogs that were undergoing regeneration of their retinotectal projections; 1 week later, the effects of implantation on the projection pattern from the optic nerve were determined. Both polyclonal and monoclonal antibodies to N-CAM distorted the retinotedal projection pattern and greatly decreased the precision of the projection; these alterations recovered to near normal after an additional 3 weeks. Similar but smaller effects were obtained when normally developing froglets received tectal implants. In control animals, implants of immunoglobulins from preimmune Serum and monoclonal antibodies not directed against N-CAM had little or no effect on the pattern. The results suggest that neuronal adhesion mediated by N-CAM is important in establishing and maintaining the precision and topography of neural patterns.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.81.13.4222DOIArticle
http://www.pnas.org/content/81/13/4222.longPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC345401/PubMed CentralArticle
http://www.jstor.org/stable/24170 JSTORArticle
ORCID:
AuthorORCID
Fraser, Scott E.0000-0002-5377-0223
Additional Information:© 1984 National Academy of Sciences. Contributed b.y Gerald M. Edelman, March 9, 1984. We thank V. Bayer for assisting in the electrophysiologic mapping experiments and Dr. S. Hoffman for useful advice. This work was supported by grants from the National Science Foundation (BNS8023638) and the U.S. Public Health Service (HD-09635, AM-04256, and AI-11378).
Funders:
Funding AgencyGrant Number
NSFBNS 8023638
U.S. Public Health Service (USPHS)HD-09635
U.S. Public Health Service (USPHS)AM-04256
U.S. Public Health Service (USPHS)AI-11378
Issue or Number:13
PubMed Central ID:PMC345401
Record Number:CaltechAUTHORS:20160520-111325950
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160520-111325950
Official Citation:S E Fraser, B A Murray, C M Chuong, and G M Edelman Alteration of the retinotectal map in Xenopus by antibodies to neural cell adhesion molecules PNAS 1984 81 (13) 4222-4226
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67205
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:20 May 2016 22:01
Last Modified:03 Oct 2019 10:03

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