Fraser, Scott E. and Murray, Ben A. and Chuong, Cheng Ming and Edelman, Gerald M. (1984) Alteration of the retinotectal map in Xenopus by antibodies to neural cell adhesion molecules. Proceedings of the National Academy of Sciences of the United States of America, 81 (13). pp. 4222-4226. ISSN 0027-8424. PMCID PMC345401. doi:10.1073/pnas.81.13.4222. https://resolver.caltech.edu/CaltechAUTHORS:20160520-111325950
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Abstract
The neural cell adhesion molecule (N-CAM) mediates neuron-neuron adhesion, is ubiquitous in the nervous system of developing and mature vertebrates, and undergoes major alterations in both amount and distribution during development. Perturbation of homophillic (N-CAM to N-CAM) building by univalent fragments of specific anti-N-CAM antibodies has previously been found to alter neural patterns in vitro. To show that significant alterations can also occur in vivo, antibodies lo Xenopus N-CAM were embedded in agarose microcylinders and implanted in the tecta of juvenile Xenopus laevis frogs that were undergoing regeneration of their retinotectal projections; 1 week later, the effects of implantation on the projection pattern from the optic nerve were determined. Both polyclonal and monoclonal antibodies to N-CAM distorted the retinotedal projection pattern and greatly decreased the precision of the projection; these alterations recovered to near normal after an additional 3 weeks. Similar but smaller effects were obtained when normally developing froglets received tectal implants. In control animals, implants of immunoglobulins from preimmune Serum and monoclonal antibodies not directed against N-CAM had little or no effect on the pattern. The results suggest that neuronal adhesion mediated by N-CAM is important in establishing and maintaining the precision and topography of neural patterns.
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Additional Information: | © 1984 National Academy of Sciences. Contributed b.y Gerald M. Edelman, March 9, 1984. We thank V. Bayer for assisting in the electrophysiologic mapping experiments and Dr. S. Hoffman for useful advice. This work was supported by grants from the National Science Foundation (BNS8023638) and the U.S. Public Health Service (HD-09635, AM-04256, and AI-11378). | |||||||||||||||
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Issue or Number: | 13 | |||||||||||||||
PubMed Central ID: | PMC345401 | |||||||||||||||
DOI: | 10.1073/pnas.81.13.4222 | |||||||||||||||
Record Number: | CaltechAUTHORS:20160520-111325950 | |||||||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160520-111325950 | |||||||||||||||
Official Citation: | S E Fraser, B A Murray, C M Chuong, and G M Edelman Alteration of the retinotectal map in Xenopus by antibodies to neural cell adhesion molecules PNAS 1984 81 (13) 4222-4226 | |||||||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||||||||
ID Code: | 67205 | |||||||||||||||
Collection: | CaltechAUTHORS | |||||||||||||||
Deposited By: | Ruth Sustaita | |||||||||||||||
Deposited On: | 20 May 2016 22:01 | |||||||||||||||
Last Modified: | 11 Nov 2021 00:28 |
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