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A long, nontranslatable poly(A) RNA stored in the egg of the sea urchin Strongylocentrotus purpuratus

Calzone, Frank J. and Lee, James J. and Le, Nga and Britten, Roy J. and Davidson, Eric H. (1988) A long, nontranslatable poly(A) RNA stored in the egg of the sea urchin Strongylocentrotus purpuratus. Genes and Development, 2 (3). pp. 305-318. ISSN 0890-9369. http://resolver.caltech.edu/CaltechAUTHORS:20160520-112154892

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Abstract

Nontranslatable transcripts containing interspersed repetitive sequence elements constitute a major fraction of the poly(A) RNA stored in the cytoplasm of both the sea urchin egg and the amphibian oocyte. We report the first complete sequence of a representative interspersed maternal RNA transcript, called ISp1. The transcript is about 3.7 kb in length [including poly(A) tail]; and the 5' half consists of a cluster of repetitive sequences, whereas the 3' half is single copy. Other repetitive sequences occur in the 5' and 3' regions flanking the transcription unit. In several cloned alleles, the flanking repetitive and single-copy sequences differ, indicating a high degree of insertional and deletional rearrangement around, as well as within, the transcription unit. No significant open reading frames exist in any region of the ISp1 transcript, nor is it spliced to give rise to translatable mRNA in egg or embryo. A 620-nucleotide repetitive sequence element at the 5' end of the ISp1 transcript is also represented in a large number of other long interspersed maternal poly(A) RNAs. In addition, this sequence appears in a prevalent set of small polyadenylated RNAs about 600-nucleotides in length, which disappear almost completely by the gastrula stage of development. The structural features of the ISp1 RNA uncovered in this work exclude several hypotheses of interspersed maternal poly(A) RNA origin and function.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1101/gad.2.3.305DOIArticle
http://genesdev.cshlp.org/content/2/3/305PublisherArticle
Additional Information:© 1988 Cold Spring Harbor Laboratory. The Authors acknowledge that six months after the full-issue publication date, the Article will be distributed under a Creative Commons CC-BY-NC License (Attribution-NonCommercial 4.0 International License, http://creativecommons.org/licenses/by-nc/4.0/). Received October 27, 1987; revised version accepted January 18, 1988. This research was supported by National Institutes of Health (NIH) grant HD-05753. F.J.C. was supported by a Lieve Senior Research Fellowship from the American Cancer Society, California Division, and J.J.L. was supported by NIH training grant GM-07616.
Funders:
Funding AgencyGrant Number
NIHHD-05753
American Cancer Society, California DivisionUNSPECIFIED
NIH Predoctoral FellowshipGM-07616
Subject Keywords:Repetitive sequence transcripts; maternal RNA; sea urchin
Record Number:CaltechAUTHORS:20160520-112154892
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160520-112154892
Official Citation:A long, nontranslatable poly(A) RNA stored in the egg of the sea urchin Strongylocentrotus purpuratus. F J Calzone, J J Lee, N Le, R J Britten, and E H Davidson Genes Dev. March 1988 2: 305-318; doi:10.1101/gad.2.3.305
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67206
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:20 May 2016 18:46
Last Modified:20 May 2016 18:46

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