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The presence of zinc in the restriction enzyme Eco RI

Barton, Jacqueline K. and Basile, Lena A. and Paranawithana, Shanthi R. (1982) The presence of zinc in the restriction enzyme Eco RI. Journal of Biological Chemistry, 257 (14). pp. 7911-7914. ISSN 0021-9258.

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We have determined that the restriction endonuclease Eco RI contains 1.0 ± 0.1 eq of zinc/monomeric enzyme. DNA cleavage by Eco RI is inhibited by ortho-phenanthroline after preincubation of the enzyme with the chelating agent. A similar inhibition by the nonchelating meta-phenanthroline is not seen. The sensitivity of the inhibition by the neutral ligand ortho-phenanthroline to preincubation is consistent with the tightly bound and inaccessible nature of the metal site. Extensive dialysis against the ortho-phenanthroline inhibitor leads to the release of the bound metal with the concomitant loss of enzyme activity. The tightly bound Zn^(2+) cation, then, appears to be necessary for enzyme function. The finding of zinc in Eco RI further illustrates the ubiquity of Zn^(2+) to DNA-protein complexes.

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Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 1982 American Society for Biochemistry and Molecular Biology. Received for publication, February 9, 1982. This work was supported by the American Chemical Society Petroleum Research Fund and the City University of New York PSCBHE Research Award Program. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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American Chemical Society Petroleum Research FundUNSPECIFIED
City University of New YorkUNSPECIFIED
Issue or Number:14
Record Number:CaltechAUTHORS:20160520-112839737
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Official Citation:J K Barton, L A Basile, and S R Paranawithana The presence of zinc in the restriction enzyme Eco RI. J. Biol. Chem. 1982 257: 7911-4.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67207
Deposited By: Tony Diaz
Deposited On:20 May 2016 18:44
Last Modified:03 Oct 2019 10:03

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