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Sequence Specific Recognition of Double Helical DNA. A Synthetic Approach

Dervan, P. B. (1988) Sequence Specific Recognition of Double Helical DNA. A Synthetic Approach. In: Nucleic Acids and Molecular Biology. Vol.2. Springer-Verlag , New York, NY, pp. 49-64. ISBN 978-3-642-83386-1. http://resolver.caltech.edu/CaltechAUTHORS:20160520-130010198

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Abstract

The design of synthetic sequence-specific DNA-binding molecules has advanced in recent years due, in part, to analytical techniques such as foot-printing and affinity cleaving which allow rapid and precise analyses of hundreds of potential DNA-binding sites to nucleotide resolution on sequencing gels (Dervan 1986). During the past 10 years our group has focused on the construction of molecules that bind in the minor and major groove of DNA with incrementally increasing sequence specificity as a first step toward defining the chemical principles for creating specificity at the 15-base pair level (Dervan 1986). Recent progress illustrates how the tools of chemical synthesis in combination with nucleic acid techniques can be used to solve this problem. This may lead to new research tools useful in molecular biology, diagnosis of disease states at the level of DNA, and novel chemotherapeutic strategies such as artificial repressors for inactivation of these genes. The purpose of this chapter is not a comprehensive survey of the literature but rather a brief report of recent progress from our group using an experimental approach to the design of sequence-specific DNA-binding molecules.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1007/978-3-642-83384-7_3DOIArticle
http://link.springer.com/chapter/10.1007%2F978-3-642-83384-7_3PublisherArticle
Additional Information:© 1988 Springer. We are grateful for the support of the National Institutes of Health, the American Cancer Society, DARPA, the National Foundation for Cancer Research, Merck, Burroughs-Wellcome, the Allied-Signal Corporation, and the Ralph M. Parsons Foundation.
Funders:
Funding AgencyGrant Number
NIHUNSPECIFIED
American Cancer SocietyUNSPECIFIED
Defense Advanced Research Projects Agency (DARPA)UNSPECIFIED
National Foundation for Cancer ResearchUNSPECIFIED
MerckUNSPECIFIED
Burroughs-WellcomeUNSPECIFIED
Allied-SignalUNSPECIFIED
Ralph M. Parsons FoundationUNSPECIFIED
Record Number:CaltechAUTHORS:20160520-130010198
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160520-130010198
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67211
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:20 May 2016 20:34
Last Modified:24 May 2016 22:58

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