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Use of Bacteriophage φX174 Replicative from Progeny DNA as Templates for Transcription

Truffaut, Nicole and Sinsheimer, Robert L. (1974) Use of Bacteriophage φX174 Replicative from Progeny DNA as Templates for Transcription. Journal of Virology, 13 (4). pp. 818-827. ISSN 0022-538X. PMCID PMC355380. https://resolver.caltech.edu/CaltechAUTHORS:20160520-142231655

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Abstract

The synthesis of φX174-specific RNA has been studied in infected cells in which the thymine of the viral (+) strand of the parental RF*, of the complementary (−) strand of the parental RF, or of both strands of the progeny RF molecules has been replaced with 5-bromouracil (5 BU). By irradiation of such cells with UV light at a wavelength of 313 nm it was possible to affect, specifically, the 5 BU-labeled strands. When the progeny RF molecules contain thymine, irradiation has no effect upon the synthesis of viral-specific RNA, regardless of 5 BU substitution in either strand of parental RF. If, however, progeny RF is labeled with 5 BU, irradiation produces a major decrease of viral RNA synthesis. It is concluded that many progeny RF molecules can serve as templates for transcription at late times of infection. Irradiation, prior to RF replication, of cells in which, particularly, the complementary strand of RF contains 5 BU, appears to decrease the ability of the parental RF to replicate.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://jvi.asm.org/content/13/4/818.abstractPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC355380/PubMed CentralArticle
Additional Information:© 1974 American Society for Microbiology. Received for publication 26 November 1973. This research was supported in part by Public Health Service grant, GM 13554, from the National Institute of General Medical Science.
Funders:
Funding AgencyGrant Number
NIHGM13554
Issue or Number:4
PubMed Central ID:PMC355380
Record Number:CaltechAUTHORS:20160520-142231655
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160520-142231655
Official Citation:Use of Bacteriophage φX174 Replicative from Progeny DNA as Templates for Transcription Nicole Truffaut and Robert L. Sinsheimer J. Virol. April 1974 13:4 818-827
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67219
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:20 May 2016 21:31
Last Modified:03 Oct 2019 10:04

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