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Nucleotide Sequence Repetition: A Rapidly Reassociating Fraction of Mouse DNA

Waring, Michael and Britten, Roy J. (1966) Nucleotide Sequence Repetition: A Rapidly Reassociating Fraction of Mouse DNA. Science, 154 (3750). pp. 791-794. ISSN 0036-8075. doi:10.1126/science.154.3750.791.

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The separated complemnentary strands of a minor component in mouse DNA reassociate with each other much more rapidly than do the complementary strands of other DNA's including those of the principal part of mouse DNA. This difference in capacity of the strands to reassociate can be used to effect a preparative separation of the minor component from the principal fraction. The rate constant for reassociation of the minor component, compared with those of viral and bacterial DNA's, indicates that the minor component consists of a short nucleotide sequence present in about one million copies.

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Additional Information:© 1966 American Association for the Advancement of Science. 23 September 1966. We thank Drs. B. Hoyer and D. Axelrod of the NIH for discussion, mouse embryos, germ-free mouse DNA, and SV40 DNA; our colleagues at the Department of Terrestrial Magnetism for discussion; and M. Chamberlin for technical assistance.
Issue or Number:3750
Record Number:CaltechAUTHORS:20160520-142711111
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Official Citation:Nucleotide Sequence Repetition: A Rapidly Reassociating Fraction of Mouse DNA Michael Waring, Roy J. Britten Science 11 Nov 1966: Vol. 154, Issue 3750, pp. 791-794 DOI: 10.1126/science.154.3750.791
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67220
Deposited By: George Porter
Deposited On:20 May 2016 21:36
Last Modified:11 Nov 2021 00:29

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