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Spatially deranged though temporally correct expression of a Strongylocentrotus purpuratus actin gene fusion in transgenic embryos of a different sea urchin family

Franks, Roberta R. and Hough-Evans, Barbara R. and Britten, Roy J. and Davidson, Eric H. (1988) Spatially deranged though temporally correct expression of a Strongylocentrotus purpuratus actin gene fusion in transgenic embryos of a different sea urchin family. Genes and Development, 2 (1). pp. 1-12. ISSN 0890-9369. http://resolver.caltech.edu/CaltechAUTHORS:20160523-070013892

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Abstract

We report the unexpected observation that cis-regulatory sequences of a Strongylocentrotus purpuratus actin gene, which direct a particular, lineage-specific pattern of embryonic expression, confer a completely different spatial pattern of expression when introduced into embryos of another sea urchin species. We utilized a fusion gene construct in which the bacterial chloramphenicol acetyl transferase (CAT) reporter gene is driven by CyIIIa actin regulatory sequences. We previously showed that the regulatory region that is included suffices to promote the accumulation of CAT mRNA in transgenic S. purpuratus embryos, on the same developmental schedule and in the same embryonic region, the aboral ectoderm, in which the CyIIIa actin gene is normally expressed (Flytzanis et al. 1987; Hough-Evans et al. 1987). When injected into zygotes of Lytechinus variegatus, which belongs to a different echinoid family, the expected temporal pattern of expression of CAT enzyme was observed. Thus, in both S. purpuratus and L. variegatus embryos, expression is activated at the early blastula stage, although this stage is attained several hours sooner in L. variegatus embryo cultures. Similar kinetics of CAT enzyme accumulation were obtained whether the gene was introduced directly into the L. variegatus zygote nucleus or into the cytoplasm. However, when examined by in situ hybridization, the transgenic L. variegatus embryos were found to display a totally new pattern of CAT mRNA accumulation. Copious CAT transcripts were detected not only in aboral ectoderm cells, but also in skeletogenic mesenchyme cells, gut cells, and oral ectoderm, all cell types that in the transgenic S. purpuratus controls are invariably devoid of detectable CAT transcripts.


Item Type:Article
Related URLs:
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http://dx.doi.org/10.1101/gad.2.1.1DOIArticle
http://genesdev.cshlp.org/content/2/1/1.abstractPublisherArticle
Additional Information:© 1988 Cold Spring Harbor Laboratory Press. The Authors acknowledge that six months after the full-issue publication date, the Article will be distributed under a Creative Commons CC-BY-NC License (Attribution-NonCommercial 4.0 International License, http://creativecommons.org/licenses/by-nc/4.0/). Received September 18, 19871 revised version accepted November 12, 1987. This research was supported by National Institutes of Health grant HD-05753. R.R.F. was supported by an NIH postdoctoral training grant HD-07257.
Funders:
Funding AgencyGrant Number
NIHHD-05753
NIH Postdoctoral FellowshipHD-07257
Subject Keywords:Gene transfer; early development; CyIIIa actin gene, spatial regulation; temporal regulation
Record Number:CaltechAUTHORS:20160523-070013892
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160523-070013892
Official Citation:Spatially deranged though temporally correct expression of Strongylocentrotus purpuratus actin gene fusion in transgenic embryos of a different sea urchin family. R R Franks, B R Hough-Evans, R J Britten, and E H Davidson Genes Dev. January 1988 2: 1-12; doi:10.1101/gad.2.1.1
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67234
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:23 May 2016 19:21
Last Modified:23 May 2016 19:21

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