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Comparative Studies of the 3'-Terrninal Sequences of Several Alphavirus RNAs

Ou, Jing-Hsiung and Strauss, Ellen G. and Strauss, James H. (1981) Comparative Studies of the 3'-Terrninal Sequences of Several Alphavirus RNAs. Virology, 109 (2). pp. 281-289. ISSN 0042-6822.

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The 3'-termini of 26 S RNA of Sindbis virus and of the virion 49 S RNAs of Sindbis virus, Semliki Forest virus, and Middelburg virus have been sequenced by using a chain-terminating sequencing method with reverse transcriptase. Both 49 S and 26 S RNAs of Sindbis virus have identical 3'-terminal sequence for at least l46 nucleotides from the poly(A) tracts. The 26 S RNA is known to be a subgenomic RNA located at or near the 3'-end of 49 S RNA; our present results indicate that 26 S and 49 S RNA are coterminal. The 49 S RNAs of all three viruses contain a region of 20 nucleotides adjacent to the poly(A) which is highly conserved. This conserved sequence could be the replicase recognition site and/or a recognition site for encapsidation. After this conserved region the sequences of Sindbis and of Semliki Forest virus diverge significantly but there does appear to be some residual sequence homology in these regions. The 3'-termini of Sindbis virus and Semliki Forest virus RNAs contain a high content of A and U; of the first 50 nucleotides adjacent to the poly(A) tail over half are U and over one quarter are A. In addition repeating sequences are present in the 3'-termini.

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Additional Information:© 1981 Academic Press, Inc. Accepted September 15th, 1980. We are grateful to E. M. Lenches for expert technical assistance, to T. E. Crowley and C. M. Rice for help in the preparation of Sindbis 26 S RNA. This work was supported by Grant PCM 77-26728 from the National Science Foundation, by Grants GM 06965 and AI-10793 from NIH, and by Biomedical Research Support Grant 507 RR 07003 from NIH. J.O. was supported by fellowships from the California Foundation for Biochemical Research and from the Li Ming Scholarship Endowment Fund.
Funding AgencyGrant Number
NSFPCM 77-26728
NIHGM 06965
NIHAI 10793
NIH507 RR 07003
California Foundation for Biochemical ResearchUNSPECIFIED
Li Ming Scholarship Endowment FundUNSPECIFIED
Issue or Number:2
Record Number:CaltechAUTHORS:20160523-153131666
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Official Citation:Jing-Hsiung Ou, Ellen G. Strauss, James H. Strauss, Comparative studies of the 3′-terminal sequences of several alphavirus RNAs, Virology, Volume 109, Issue 2, 1981, Pages 281-289, ISSN 0042-6822, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67267
Deposited By: Donna Wrublewski
Deposited On:24 May 2016 00:02
Last Modified:03 Oct 2019 10:04

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