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Regulation of Meristem Morphogenesis by Cell Wall Synthases in Arabidopsis

Yang, Weibing and Schuster, Christoph and Beahan, Cherie T. and Charoensawan, Varodom and Peaucelle, Alexis and Bacic, Antony and Doblin, Monika S. and Wightman, Raymond and Meyerowitz, Elliot M. (2016) Regulation of Meristem Morphogenesis by Cell Wall Synthases in Arabidopsis. Current Biology, 26 (11). pp. 1404-1415. ISSN 0960-9822. PMCID PMC5024349. doi:10.1016/j.cub.2016.04.026. https://resolver.caltech.edu/CaltechAUTHORS:20160525-090435334

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Abstract

The cell walls of the shoot apical meristem (SAM), containing the stem cell niche that gives rise to the above-ground tissues, are crucially involved in regulating differentiation. It is currently unknown how these walls are built and refined or their role, if any, in influencing meristem developmental dynamics. We have combined polysaccharide linkage analysis, immuno-labeling, and transcriptome profiling of the SAM to provide a spatiotemporal plan of the walls of this dynamic structure. We find that meristematic cells express only a core subset of 152 genes encoding cell wall glycosyltransferases (GTs). Systemic localization of all these GT mRNAs by in situ hybridization reveals members with either enrichment in or specificity to apical subdomains such as emerging flower primordia, and a large class with high expression in dividing cells. The highly localized and coordinated expression of GTs in the SAM suggests distinct wall properties of meristematic cells and specific differences between newly forming walls and their mature descendants. Functional analysis demonstrates that a subset of CSLD genes is essential for proper meristem maintenance, confirming the key role of walls in developmental pathways.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.cub.2016.04.026DOIArticle
http://www.sciencedirect.com/science/article/pii/S0960982216303487PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5024349/PubMed CentralArticle
ORCID:
AuthorORCID
Yang, Weibing0000-0002-2379-5729
Schuster, Christoph0000-0002-1948-2367
Peaucelle, Alexis0000-0002-8582-6500
Wightman, Raymond0000-0003-1295-4875
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2016 Elsevier Ltd. Received: March 11, 2016; Revised: March 24, 2016; Accepted: April 11, 2016; Published: May 19, 2016. The authors wish to thank Zachary Nimchuk for advice on reporter construction, Arun Sampathkumar for help with 3D visualization of the GALT31A reporter, and Lisa Willis for help with spherical cap calculations. V.C. is a recipient of a Thailand Research Fund grant for new researchers (grant TRG5880067) and a research supplement grant from the Faculty of Science, Mahidol University. C.T.B., M.S.D., and A.B. acknowledge the support of the ARC Centre of Excellence in Plant Cell Walls (grant CE110001007). E.M.M. acknowledges support from the Gatsby Charitable Trust through fellowships GAT3272/C and GAT3273-PR1, Howard Hughes Medical Institute, Gordon and Betty Moore Foundation (through grant GBMF3406), and U.S. Department of Energy (through award DE-FG02-99ER13873). A.P. acknowledges support of the EU Marie-Curie FP7 COFUND People Programme through the award of an AgreenSkills (grant 267196). R.W. acknowledges support from the Leverhulme Trust (grant RPG-2015-285). Author Contributions: W.Y. and R.W. generated the cell wall preparations and carried out morphological analysis of the csld mutants; W.Y. carried out the RNA in situ hybridizations with the help of C.S. and R.W.; C.S. prepared the RNA-seq libraries and carried out the GO analysis; C.S. and V.C. processed the RNA-seq data; C.T.B., A.B., and M.S.D. carried out the linkage analysis and calculated the polysaccharide composition; A.P. devised and performed the cell wall immuno-labeling protocols; R.W. and M.S.D. carried out the literature searches, identified meristem glycosyltransferases, and amalgamated the chemical and expression data; W.Y. and R.W. prepared the figures; and R.W., M.S.D., E.M.M., and A.B. devised the project and wrote the manuscript with contributions from the remaining authors.
Funders:
Funding AgencyGrant Number
Thailand Research FundTRG5880067
Mahidol UniversityUNSPECIFIED
Australian Research CouncilCE110001007
Gatsby Charitable TrustGAT3272/C
Gatsby Charitable TrustGAT3273-PR1
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Gordon and Betty Moore FoundationGBMF3406
Department of Energy (DOE)DE-FG02-99ER13873
Marie Curie Fellowship267196
Leverhulme TrustRPG-2015-285
Issue or Number:11
PubMed Central ID:PMC5024349
DOI:10.1016/j.cub.2016.04.026
Record Number:CaltechAUTHORS:20160525-090435334
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160525-090435334
Official Citation:Weibing Yang, Christoph Schuster, Cherie T. Beahan, Varodom Charoensawan, Alexis Peaucelle, Antony Bacic, Monika S. Doblin, Raymond Wightman, Elliot M. Meyerowitz, Regulation of Meristem Morphogenesis by Cell Wall Synthases in Arabidopsis, Current Biology, Volume 26, Issue 11, 6 June 2016, Pages 1404-1415, ISSN 0960-9822, http://dx.doi.org/10.1016/j.cub.2016.04.026. (http://www.sciencedirect.com/science/article/pii/S0960982216303487)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67339
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:26 May 2016 19:49
Last Modified:11 Nov 2021 00:31

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