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Studies upon DNA synthesis during multiplicity reactivation of T2r^+ bacteriophage

Nutter, Robert L. and Sinsheimer, Robert L. (1959) Studies upon DNA synthesis during multiplicity reactivation of T2r^+ bacteriophage. Virology, 7 (3). pp. 276-290. ISSN 0042-6822. doi:10.1016/0042-6822(59)90198-9.

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Studies of the synthesis of bacteriophage T2r^+ deoxyribonucleic acid (DNA) in bacterial cells multiply infected with either ultraviolet (uv)-irradiated or normal T2r^+ particles indicate that there is a lag of 8–10 minutes in the production of viral DNA in the former case as compared to the latter. Premature lysis of the cells reveals that a similar 8–10 minute lag is present in the appearance of active intracellular phage in these instances. This result is observed using conditions in which multiplicity reactivation can be demonstrated. By using 5-methyltryptophan as an inhibitor of protein synthesis in cultures of phage-infected cells, it was determined that protein synthesis is necessary soon after infection with uv-irradiated as well as with unirradiated T2r^+ in order for production of phage DNA to take place. Protein synthesis is necessary for the initiation of DNA synthesis, but not for its continuation. Evidence was obtained that some of the steps in the multiplicity reactivation process, subsequent to the initial steps, may not require protein synthesis.

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Additional Information:© 1959 Elsevier Inc. ccepted 24 November 1958. This research was supported by grants from the American Cancer Society and the United States Public Health Service. This manuscript is adapted in part from a thesis submitted by R. L. N. in partial fulfillment of the requirement for the Ph.D. at Iowa State College.
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American Cancer SocietyUNSPECIFIED
U.S. Public Health Service (USPHS)UNSPECIFIED
Issue or Number:3
Record Number:CaltechAUTHORS:20160527-121103102
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Official Citation:Robert L. Nutter, Robert L. Sinsheimer, Studies upon DNA synthesis during multiplicity reactivation of T2r+ bacteriophage, Virology, Volume 7, Issue 3, 1959, Pages 276-290, ISSN 0042-6822, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67455
Deposited By: Ruth Sustaita
Deposited On:27 May 2016 19:49
Last Modified:11 Nov 2021 00:32

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