Lunan, Kenneth D. and Sinsheimer, Robert L. (1956) A study of the nucleic acid of bacteriophage T7. Virology, 2 (4). pp. 455-462. ISSN 0042-6822. doi:10.1016/0042-6822(56)90003-4. https://resolver.caltech.edu/CaltechAUTHORS:20160531-071558403
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Abstract
A T7 bacteriophage preparation has been obtained with a P per infective particle of 8.2 × 10^(−18) g. Only 71% of the P of the preparation is present as DNA. No RNA is present. DNA was prepared from T7 in 70% yield by denaturation of the virus with urea plus dodecyl sulfate, followed by deproteinization by chloroform. The molar proportions of bases in the DNA are: deoxyadenylic acid, 1.04; thymidylic acid, 1.04; deoxycytidylic acid, 0.96; deoxyguanylic acid, 0.96. During complete digestion of the T7 DNA with pancreatic deoxyribonuclease, 19.4% of the phosphate diester bonds were split. After fractionation of the digest by ion-exchange chromatography, 5.9% of the P of the digest was recovered as dinucleotides. These were fractionated and their relative proportions determined. Four of the dinucleotide fractions were analyzed for their isomeric position.
| Item Type: | Article | |||||||||
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| Additional Information: | © 1956 Elsevier Inc. Accepted 7 May 1956. This research was supported by grants from the American Cancer Society, acting through the Committee on Growth of the National Research Council, and from the National Cancer Institute of the National Institutes of Health, U.S. Public Health Service. | |||||||||
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| Issue or Number: | 4 | |||||||||
| DOI: | 10.1016/0042-6822(56)90003-4 | |||||||||
| Record Number: | CaltechAUTHORS:20160531-071558403 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160531-071558403 | |||||||||
| Official Citation: | Kenneth D. Lunan, Robert L. Sinsheimer, A study of the nucleic acid of bacteriophage T7, Virology, Volume 2, Issue 4, 1956, Pages 455-462, ISSN 0042-6822, http://dx.doi.org/10.1016/0042-6822(56)90003-4. (http://www.sciencedirect.com/science/article/pii/0042682256900034) | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 67466 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Ruth Sustaita | |||||||||
| Deposited On: | 31 May 2016 23:45 | |||||||||
| Last Modified: | 11 Nov 2021 00:32 |
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