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Partition Cell Analysis of Infective Tobacco Mosaic Virus Nucleic Acid

Friesen, Benjamin S. and Sinsheimer, Robert L. (1959) Partition Cell Analysis of Infective Tobacco Mosaic Virus Nucleic Acid. Journal of Molecular Biology, 1 (4-5). pp. 321-328. ISSN 0022-2836. https://resolver.caltech.edu/CaltechAUTHORS:20160531-072823483

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Abstract

The infective principle of ribonucleic acid (RNA) isolated from tobacco mosaic virus (TMV), either by Duponol C or water-saturated phenol, has a sedimentation constant of 19·6 Svedberg units at 5°C in 0·02 M-phosphate, pH 7·3, as determined by partition cell ultracentrifugal analysis. This corresponds to an S_(20) of 30. In fresh samples of RNA, the bulk of the material has the above sedimentation rate. The weight average molecular weight of such samples is 2 × 10^6 as determined by light scattering. The sedimentation rate of the infective principle in samples degraded at room temperature to various fractions of residual activity remains the same, although the bulk of the material sediments at a much slower rate. It is therefore concluded that the smaller components do not carry a detectable amount of the infectivity.


Item Type:Article
Related URLs:
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http://dx.doi.org/10.1016/S0022-2836(59)80013-9DOIArticle
http://www.sciencedirect.com/science/article/pii/S0022283659800139PublisherArticle
Additional Information:© 1959 Elsevier Ltd. Received 13 July 1959. The research reported in this paper was performed at the California Institute of Technology, but also formed a major part of the Ph.D. dissertation of B. S. F., submitted to Iowa State College. The research reported in this paper was supported in part by grants from the American Cancer Society, U.S. Public Health Service and the California Division of the American Cancer Society. It is a pleasure to acknowledge the able technical assistance of Miss Barbara Starman and Mr. Robert Bradford. Financial support to one of us (B. S. F.) from the Arthur McCollum Fund is gratefully acknowledged.
Funders:
Funding AgencyGrant Number
American Cancer SocietyUNSPECIFIED
U.S. Public Health Service (USPHS)UNSPECIFIED
American Cancer Society, California DivisionUNSPECIFIED
Arthur McCollum FundUNSPECIFIED
Issue or Number:4-5
Record Number:CaltechAUTHORS:20160531-072823483
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160531-072823483
Official Citation:Benjamin S. Friesen, Robert L. Sinsheimer, Partition cell analysis of infective tobacco mosaic virus nucleic acid, Journal of Molecular Biology, Volume 1, Issue 4, 1959, Pages 321-328, ISSN 0022-2836, http://dx.doi.org/10.1016/S0022-2836(59)80013-9. (http://www.sciencedirect.com/science/article/pii/S0022283659800139)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67467
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:31 May 2016 23:42
Last Modified:03 Oct 2019 10:06

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