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A Deoxyribonuclease from Calf Spleen: I Purification and Properties

Koerner, James F. and Sinsheimer, Robert L. (1957) A Deoxyribonuclease from Calf Spleen: I Purification and Properties. Journal of Biological Chemistry, 228 (2). pp. 1039-1048. ISSN 0021-9258.

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The deoxyribonucleases1 of animal tissues are of two types which are readily distinguishable by the conditions necessary for activity on their substrate, DNA. The pancreatic DNase, which has been crystallized by Kunitz (1), is active in neutral solution in the presence of magnesium or certain other divalent cations (2). The DN ase predominant in most other tissues is active at a lower pH, in the presence of adequate ionic strength, but does not specifically require divalent ions.

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Additional Information:© 1957 American Society for Biochemistry and Molecular Biology. Received for publication, February 18, 1957. The research described in this paper was supported by a grant from the National Cancer Institute of the United States Public Health Service. Portions of this manuscript are taken from a dissertation submitted by J.F. Koerner in partial fulfilment of the requirements for the degree of Doctor of Philosophy at Iowa State College.
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National Cancer InstituteUNSPECIFIED
U.S. Public Health Service (USPHS)UNSPECIFIED
Issue or Number:2
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Official Citation:A DEOXYRIBONUCLEASE FROM CALF SPLEEN: I. PURIFICATION AND PROPERTIES James F. Koerner and Robert L. Sinsheimer J. Biol. Chem. 1957 228: 1039.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67468
Deposited On:31 May 2016 23:25
Last Modified:03 Oct 2019 10:06

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