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A Purification of venom phosphodiesterase

Sinsheimer, Robert L. and Koerner, James F. (1952) A Purification of venom phosphodiesterase. Journal of Biological Chemistry, 198 (1). pp. 293-296. ISSN 0021-9258. https://resolver.caltech.edu/CaltechAUTHORS:20160531-085521801

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Abstract

For purposes of analysis of polynucleotides, it is desirable to have a phosphodiesterase, substantially free of 5-nucleotidase or other phosphatase activity. The presence of a phosphodiesterase in a wide variety of snake venoms was demonstrated by Gullan and Jackson (1). These venoms were found also to contain a potent 5-nucleotidase, but were free of alkaline phosphatase activity. Hurst and Butler (2) found that certain samples of Russell's viper venom were nearly free of 5-nucleotidase activity, while retaining potent phosphodiesterase action. By a chromatographic procedure, involving the use of cellulose columns, they were able to reduce the 5-nucleotidase activity of rattlesnake venom, relative to its phosphodiesterase activity, and to obtain fractions nearly comparable to the viper venom.


Item Type:Article
Related URLs:
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http://www.jbc.org/content/198/1/293.shortPublisherArticle
Additional Information:© 1952 American Society for Biochemistry and Molecular Biology. Received for publication, May 5, 1952. This work was supported by a grant from the Rockefeller Foundation.
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Funding AgencyGrant Number
Rockefeller FoundationUNSPECIFIED
Issue or Number:1
Record Number:CaltechAUTHORS:20160531-085521801
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160531-085521801
Official Citation:A PURIFICATION OF VENOM PHOSPHODIESTERASE Robert L. Sinsheimer and James F. Koerner J. Biol. Chem. 1952 198: 293.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67480
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:31 May 2016 23:33
Last Modified:03 Oct 2019 10:06

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