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A Deoxyribonuclease from Calf Spleen. II. Mode of Action

Koerner, James F. and Sinsheimer, Robert L. (1957) A Deoxyribonuclease from Calf Spleen. II. Mode of Action. Journal of Biological Chemistry, 228 (2). pp. 1049-1062. ISSN 0021-9258.

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When highly polymerized deoxyribonucleic acid is digested with pancreatic DNase and the digest further degraded with phosphodiesterase from intestinal mucose or snake venom, the mononucleotides formed are 5'-phosphates. Since a digest prepared in this manner with a purified venom diesterase contains only 5'-mononucleotides and practically no nucleosides or polynucleotides, the pancreatic DNase must form only polynucleotides with 5' monoesterified phosphate end groups. Recently the enzymatic synthesis of polydeoxyribonucleotides from eoxyribonucleoside 5'-triphosphates has been demonstrated. Thus both enzymatic synthesis and degradation of deoxyribonucleic acids have as yet involved only derivatives with 5'-monoesterified phosphate.

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Additional Information:© 1957 American Society for Biochemistry and Molecular Biology. Received for publication, February 18, 1957. The research described in this paper was supported by a grant from the National Cancer Institute of the United States Public Health Service.
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National Cancer InstituteUNSPECIFIED
U.S. Public Health Service (USPHS)UNSPECIFIED
Issue or Number:2
Record Number:CaltechAUTHORS:20160531-092951751
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Official Citation:A DEOXYRIBONUCLEASE FROM CALF SPLEEN: II. MODE OF ACTION James F. Koerner and Robert L. Sinsheimer J. Biol. Chem. 1957 228: 1049-1062
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67484
Deposited On:31 May 2016 23:31
Last Modified:03 Oct 2019 10:06

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