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Ultraviolet Absorption Spectra at Reduced Temperatures. I. Principles and Methods

Sinsheimer, R. L. and Scott, J. F. and Loofbourow, J. R. and Best, B. and Hastings, R. and Vollmer, E. and Westergaard, M. (1950) Ultraviolet Absorption Spectra at Reduced Temperatures. I. Principles and Methods. Journal of Biological Chemistry, 187 (1). pp. 299-312. ISSN 0021-9258.

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Low temperature absorption and fluorescence spectra of solids, liquids, and solutions often reveal increased spectral detail of use in analytical procedures and molecular structure studies. Nevertheless, while qualitative observations of the influence of liquid air temperatures upon optical properties were undertaken very early, investigations of the absorption and fluorescence of organic compounds at the temperature of liquid nitrogen (-195.6°; 77.4 °K.) and below have appeared only sporadically. Because of the potential usefulness of the technique we have undertaken a systematic study of the low temperature spectra of substances of biochemical interest. The present paper discusses the methods employed; subsequent papers will deal with the experimental results. In this work, we have emphasized the wave-length location of absorption bands and the accurate determination of relative optical densities rather than precision in the determination of absolute optical densities, thus permitting the use of simpler methods than would otherwise be necessary.

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Additional Information:© 1950 American Society for Biochemistry and Molecular Biology. Received for publication, April 24, 1950. Supported in part by a grant from the American Cancer Society, upon recommendation of the Committee on Growth of the National Research Council.
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American Cancer SocietyUNSPECIFIED
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Official Citation:ULTRAVIOLET ABSORPTION SPECTRA AT REDUCED TEMPERATURES: I. PRINCIPLES AND METHODS R. L. Sinsheimer, J. F. Scott, J. R. Loofbourow, and With the technical assistance of B. Best, R. Hastings, E. Vollmer, and M. Westergaard J. Biol. Chem. 1950 187: 299-312.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67486
Deposited By: Ruth Sustaita
Deposited On:31 May 2016 23:29
Last Modified:03 Oct 2019 10:06

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