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The process of infection with bacteriophage φX174: XXVII. Synthesis of a viral-specific chloramphenicol-resistant protein in φX174-infected cells

Levine, Arnold J. and Sinsheimer, Robert L. (1969) The process of infection with bacteriophage φX174: XXVII. Synthesis of a viral-specific chloramphenicol-resistant protein in φX174-infected cells. Journal of Molecular Biology, 39 (3). pp. 655-668. ISSN 0022-2836. https://resolver.caltech.edu/CaltechAUTHORS:20160531-120750836

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Abstract

Cistron VI of φX174 codes for a chloramphenicol-resistant protein that is required for the synthesis of progeny replicative form. The gene product of cistron VI has been isolated by CaHPO_4 chromatography using a double label technique in the presence of 30 μg of CM‡/ml. (Levine & Sinsheimer, 1968a). This technique has been used to demonstrate that the kinetics of synthesis of cistron VI protein are similar to the kinetics of synthesis of progeny RF DNA. The molar quantities of this protein made in 30 μg of CM/ml. are approximately equal to the molar amounts of progeny RF made under these same conditions. Mutants in the six other known cistrons of φX174 synthesize about the same quantities of cistron VI protein as does wild-type φX in 30 μg of CM/ml. A study of the intracellular distribution of cistron VI protein demonstrated that this CM-resistant protein is first found associated with a rapidly sedimenting cellular component (along with the parental RF) and at later times is found in the more slowly sedimenting soluble protein fraction. In Rep− cells, which do not synthesize progeny RF, cistron VI protein is synthesized and is found associated only with the rapidly sedimenting fraction.


Item Type:Article
Related URLs:
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http://dx.doi.org/10.1016/0022-2836(69)90151-XDOIArticle
http://www.sciencedirect.com/science/article/pii/002228366990151XPublisherArticle
Additional Information:© 1969 Elsevier Ltd. Received 18 July 1968. One of us (A. J. L.) was a postdoctoral fellow of the U.S. Public Health Service. This research was supported in part by grant GM13554 from the U.S. Public Health Service.
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Funding AgencyGrant Number
U.S. Public Health Service (USPHS)GM13554
NIH Postdoctoral FellowshipUNSPECIFIED
Issue or Number:3
Record Number:CaltechAUTHORS:20160531-120750836
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160531-120750836
Official Citation:Arnold J. Levine, Robert L. Sinsheimer, The process of infection with bacteriophage φX174, Journal of Molecular Biology, Volume 39, Issue 3, 1969, Pages 655-668, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(69)90151-X. (http://www.sciencedirect.com/science/article/pii/002228366990151X)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67496
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:31 May 2016 23:23
Last Modified:03 Oct 2019 10:06

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