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Lysis of Escherichia coli with a neutral detergent

Godson, G. Nigel and Sinsheimer, Robert L. (1967) Lysis of Escherichia coli with a neutral detergent. Biochimica et Biophysica Acta, 149 (2). pp. 476-488. ISSN 0006-3002.

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1. When Escherichia coli cells are treated with lysozyme and versene for 30–45 sec in an ice-bath, they become susceptible to lysis by the neutral detergent Brij-58. 2. The rate of lysis is dependent upon the concentration of the detergent. Brij-58 is approx. 1000-fold less active in lysis of the lysozyme-versene-treated cells than is deoxycholic acid or sodium docecylsulphate. 3. The extent of cell disruption is dependent upon the Mg^(2+) concentration and the ionic strength at the time of lysis. In 70 mM Mg^(2+), only the low molecular weight soluble RNA and soluble protein are released from the cell. In 40 mM Mg^(2+), the 70-S monosomes and the ribosome subunits are released together with the soluble material. At still lower Mg^(2+) concentrations, the polyribosomes are released. 4. Unless the Mg^(2+) concentration is reduced below 5 mM, the cellular DNA, after lysis, may be almost completely sedimented by centrifugation at 3000 × g for 5 min. 5. All strains of E. coli tested were susceptible to lysis by this method.

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Additional Information:© 1967 Elsevier B.V. Received June 19th, 1967. This research was supported, in part, by Grants GR6965 and GM13554 from the U.S. Public Health Service.
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)GR 6965
U.S. Public Health Service (USPHS)GM13554
Issue or Number:2
Record Number:CaltechAUTHORS:20160531-130346235
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Official Citation:G.Nigel Godson, Robert L. Sinsheimer, Lysis of Escherichia coli with a neutral detergent, Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, Volume 149, Issue 2, 1967, Pages 476-488, ISSN 0005-2787, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67497
Deposited By: Ruth Sustaita
Deposited On:31 May 2016 23:17
Last Modified:03 Oct 2019 10:06

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