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Lysis of Escherichia coli with a neutral detergent

Godson, G. Nigel and Sinsheimer, Robert L. (1967) Lysis of Escherichia coli with a neutral detergent. Biochimica et Biophysica Acta, 149 (2). pp. 476-488. ISSN 0006-3002. http://resolver.caltech.edu/CaltechAUTHORS:20160531-130346235

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Abstract

1. When Escherichia coli cells are treated with lysozyme and versene for 30–45 sec in an ice-bath, they become susceptible to lysis by the neutral detergent Brij-58. 2. The rate of lysis is dependent upon the concentration of the detergent. Brij-58 is approx. 1000-fold less active in lysis of the lysozyme-versene-treated cells than is deoxycholic acid or sodium docecylsulphate. 3. The extent of cell disruption is dependent upon the Mg^(2+) concentration and the ionic strength at the time of lysis. In 70 mM Mg^(2+), only the low molecular weight soluble RNA and soluble protein are released from the cell. In 40 mM Mg^(2+), the 70-S monosomes and the ribosome subunits are released together with the soluble material. At still lower Mg^(2+) concentrations, the polyribosomes are released. 4. Unless the Mg^(2+) concentration is reduced below 5 mM, the cellular DNA, after lysis, may be almost completely sedimented by centrifugation at 3000 × g for 5 min. 5. All strains of E. coli tested were susceptible to lysis by this method.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0005-2787(67)90175-XDOIArticle
http://www.sciencedirect.com/science/article/pii/000527876790175XPublisherArticle
Additional Information:© 1967 Elsevier B.V. Received June 19th, 1967. This research was supported, in part, by Grants GR6965 and GM13554 from the U.S. Public Health Service.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)GR 6965
U.S. Public Health Service (USPHS)GM13554
Record Number:CaltechAUTHORS:20160531-130346235
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160531-130346235
Official Citation:G.Nigel Godson, Robert L. Sinsheimer, Lysis of Escherichia coli with a neutral detergent, Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, Volume 149, Issue 2, 1967, Pages 476-488, ISSN 0005-2787, http://dx.doi.org/10.1016/0005-2787(67)90175-X. (http://www.sciencedirect.com/science/article/pii/000527876790175X)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67497
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:31 May 2016 23:17
Last Modified:31 May 2016 23:17

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