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Use of Brij lysis as a general method to prepare polyribosomes from Escherichia coli

Godson, G. Nigel and Sinsheimer, Robert L. (1967) Use of Brij lysis as a general method to prepare polyribosomes from Escherichia coli. Biochimica et Biophysica Acta, 149 (2). pp. 489-495. ISSN 0006-3002. http://resolver.caltech.edu/CaltechAUTHORS:20160531-144135326

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Abstract

1. A method of preparing polyribosomes from any strain of Escherichia coli is described. 2. The polyribosome patterns obtained vary with the growth rate (growth media) of the cell. When the cells are grown (in rich media) with a short generation time, there are very few 30-S and 50-S ribosome subunits; when the cells are grown (in a poor medium) with a long generation time, the relative proportion of ribosome subunits increases greatly. 3. Under particular growth conditions, the polyribosome pattern varies with the strain of cell used.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0005-2787(67)90176-1DOIArticle
http://www.sciencedirect.com/science/article/pii/0005278767901761PublisherArticle
Additional Information:© 1967 Elsevier B.V. Received 19 June 1967. This research was supported in part by Grants RG6965 and GM13554 of the U.S. Public Health Service.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)RG6965
U.S. Public Health Service (USPHS)GM13554
Record Number:CaltechAUTHORS:20160531-144135326
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160531-144135326
Official Citation:G.Nigel Godson, Robert L. Sinsheimer, Use of Brij lysis as a general method to prepare polyribosomes from Escherichia coli, Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, Volume 149, Issue 2, 1967, Pages 489-495, ISSN 0005-2787, http://dx.doi.org/10.1016/0005-2787(67)90176-1. (http://www.sciencedirect.com/science/article/pii/0005278767901761)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67500
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:31 May 2016 23:08
Last Modified:31 May 2016 23:08

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