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The Replication of Bacteriophage MS2: IX. Structure and Replication of the Replicative Intermediate

Kelly, Regis B. and Sinsheimer, Robert L. (1967) The Replication of Bacteriophage MS2: IX. Structure and Replication of the Replicative Intermediate. Journal of Molecular Biology, 29 (2). pp. 237-249. ISSN 0022-2836.

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Experiments are described to determine whether the ribonuclease-sensitive component of the replicative intermediate formed during RNA bacteriophage infection is the nascent strand (conservative replication), or is a displaced portion of the viral strand of the RNA duplex (semi-conservative replication). Appropriate labeling experiments indicate that this component arises in each replicative intermediate molecule, with equal probability, from either origin. Those replicative intermediate molecules in which the nascent strand is ribonuclease-sensitive are more readily denatured than those in which the viral strand is partially displaced. The two types of replicative intermediate can be distinguished on this basis. It is proposed that each replicative intermediate molecule replicates both conservatively and semi-conservatively, equally often.

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Additional Information:© 1967 Elsevier Ltd. Received 28 April 1967. This research was supported, in part, by grant GM13554 from the U.S. Public Health Service.
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U.S. Public Health Service (USPHS)GM13554
Issue or Number:2
Record Number:CaltechAUTHORS:20160531-152322587
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Official Citation:Regis B. Kelly, Robert L. Sinsheimer, The replication of bacteriophage MS2, Journal of Molecular Biology, Volume 29, Issue 2, 1967, Pages 237-249, ISSN 0022-2836, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67506
Deposited By: Ruth Sustaita
Deposited On:01 Jun 2016 21:13
Last Modified:03 Oct 2019 10:06

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