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The Process of Infection with Bacteriophage φX174: XV. Bacteriophage DNA Synthesis in Abortive Infections with a Set of Conditional Lethal Mutants

Lindqvist, Björn H. and Sinsheimer, Robert L. (1967) The Process of Infection with Bacteriophage φX174: XV. Bacteriophage DNA Synthesis in Abortive Infections with a Set of Conditional Lethal Mutants. Journal of Molecular Biology, 30 (1). pp. 69-80. ISSN 0022-2836. http://resolver.caltech.edu/CaltechAUTHORS:20160601-083657449

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Abstract

Mitomycin C treatment of a mutant bacterial strain defective in its host-cell reactivation system will effectively suppress bacterial DNA synthesis while at the same time allowing normal growth of bacteriophage φX174. In contrast, mitomycin C-treated wild-type cells will not support φX growth. Using such mitomycin C-treated HCR− cells, it has been possible to study the incorporation of tritiated thymidine (or thymine) into viral DNA during abortive infections with several conditional lethal mutants of φX. Mutants representing each of the six known complementation groups have been studied. It has been found that mutants from three of the six groups form and reproduce the replicative form but are unable to synthesize infective or non-infective progeny single-stranded DNA. Mutants from two groups form and reproduce replicative form and also produce single-stranded progeny DNA. Mutant strains from one group form, but are unable to replicate, replicative form; nor do they synthesize single-stranded viral DNA.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0022-2836(67)90244-6DOIArticle
http://www.sciencedirect.com/science/article/pii/0022283667902446PublisherArticle
Additional Information:© 1967 Elsevier Ltd. Received 20 June 1967. This research was supported in part by grant GM 13554 from the U.S. Public Health Service. One of us (B. H. L.) acknowledges a U.S. Government travel grant under the Fulbright-Hays Act. A preliminary account of this work was presented at the annual meeting of the Federation of American Societies for Experimental Biology in Atlantic City in 1966.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)GM13554
Fulbright CommissionUNSPECIFIED
Record Number:CaltechAUTHORS:20160601-083657449
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160601-083657449
Official Citation:Björn H. Lindqvist, Robert L. Sinsheimer, The process of infection with bacteriophage φX174, Journal of Molecular Biology, Volume 30, Issue 1, 1967, Pages 69-80, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(67)90244-6. (http://www.sciencedirect.com/science/article/pii/0022283667902446)
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ID Code:67520
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:01 Jun 2016 21:08
Last Modified:01 Jun 2016 21:08

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