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The Process of Infection with Bacteriophage φX174: XVII. Effects of Specific Metabolic Interruptions

Greenlee, Lorance L. and Sinsheimer, Robert L. (1968) The Process of Infection with Bacteriophage φX174: XVII. Effects of Specific Metabolic Interruptions. Journal of Molecular Biology, 32 (2). pp. 303-320. ISSN 0022-2836. doi:10.1016/0022-2836(68)90011-9.

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The effects of metabolic interruptions on the infective process of bacteriophage φX174 in Escherichia coli THU (a derivative of E. coli 15, requiring thymine, histidine and uracil) have been studied. The experiments indicate that: 1.(1) the synthesis of a histidine-containing protein is necessary for the replication of the parental replicative form φX DNA; 2.(2) the continued synthesis of viral DNA is necessary to maintain a normal rate of synthesis of antiserum-precipitable viral protein; in the absence of thymidine the rate of synthesis of this protein falls off progressively; and 3.(3) there appears to be a requirement for concomitant protein and DNA synthesis to produce mature virus particles. The nature of the concomitant protein requirement is not known although it does not appear to be the anti-serum-precipitable protein.

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Additional Information:© 1968 Elsevier Ltd. Received 4 August 1967, Revised 6 November 1967. This research was supported in part by grants RG6965 and GM13554 from the U.S. Public Health Service. One of us (L. L. G.) was a National Science Foundation post-doctoral fellow from 1963 to 1965.
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)RG6965
U.S. Public Health Service (USPHS)GM13554
NSF Postdoctoral FellowshipUNSPECIFIED
Issue or Number:2
Record Number:CaltechAUTHORS:20160601-085450671
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Official Citation:Lorance L. Greenlee, Robert L. Sinsheimer, The process of infection with bacteriophage φX174, Journal of Molecular Biology, Volume 32, Issue 2, 1968, Pages 303-320, ISSN 0022-2836, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67523
Deposited By: Ruth Sustaita
Deposited On:01 Jun 2016 21:03
Last Modified:11 Nov 2021 03:50

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