Kiger, John A., Jr. and Young, Elton T. and Sinsheimer, Robert L. (1968) Purification and properties of Intracellular Lamba DNA Rings. Journal of Molecular Biology, 33 (2). pp. 395-413. ISSN 0022-2836. doi:10.1016/0022-2836(68)90197-6. https://resolver.caltech.edu/CaltechAUTHORS:20160601-085450996
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Abstract
Intracellular lambda DNA has been purified from bacteria infected with lambda phage. The prediction from sedimentation properties that one intracellular form of lambda DNA is a twisted, closed-circular molecule has been confirmed by observation in the electron microscope. Two unique forms of lambda DNA are present in infected cells: twisted, closed-circular molecules (component I) and open-circular molecules (component II). One single-strand scission is sufficient to convert component I to component II. Both components I and II, either native or denatured, are able to infect spheroplasts. The infective entity present after denaturation of component I is a molecule in which strand separation has not occurred. That present after denaturation of component II is a single-strand ring; single-stranded linear molecules are not infective. The ionic strength dependence of the sedimentation rate of components I and II and phage lambda DNA has been studied. For comparative purposes, similar studies have been made with φX174 replicative form. A new consequence of DNA circularity is evident from these studies.
| Item Type: | Article | |||||||||
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| Additional Information: | © 1968 Elsevier Ltd. Received 26 September 1967. This research was supported in part by grant GM13554 from the U.S. Public Health Service. | |||||||||
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| Issue or Number: | 2 | |||||||||
| DOI: | 10.1016/0022-2836(68)90197-6 | |||||||||
| Record Number: | CaltechAUTHORS:20160601-085450996 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160601-085450996 | |||||||||
| Official Citation: | John A. Kiger, Elton T. Young, Robert L. Sinsheimer, Purification and properties of intracellular lamba DNA rings, Journal of Molecular Biology, Volume 33, Issue 2, 1968, Pages 395-413, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(68)90197-6. (http://www.sciencedirect.com/science/article/pii/0022283668901976) | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 67524 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Ruth Sustaita | |||||||||
| Deposited On: | 01 Jun 2016 21:05 | |||||||||
| Last Modified: | 11 Nov 2021 03:50 |
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