Knippers, Rolf and Sinsheimer, Robert L. (1968) Process of infection with bacteriophage φX174: XX. Attachment of the Parental DNA of Bacteriophage φX174 to a Fast-sedimenting Cell Component. Journal of Molecular Biology, 34 (1). pp. 17-29. ISSN 0022-2836. doi:10.1016/0022-2836(68)90231-3. https://resolver.caltech.edu/CaltechAUTHORS:20160601-093439043
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Abstract
The parental viral DNA of bacteriophage φX174 is found attached to a cellular structural component in gently lysed φX-am3-infected host cells. The attached RF DNA replicates semiconservatively, releasing the replication product into the cytoplasm of the cell, Cytoplasmic RF DNA does not replicate semiconservatively. The attached viral DNA can be removed from the cellular component by detergents, high pH or pronase digestion. Part of the attached φX DNA is RFII; the cytoplasmic RF (in chloramphenicol-treated cells) is exclusively RFI. The nature of the cellular component is unknown.
| Item Type: | Article | |||||||||
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| Additional Information: | © 1968 Elsevier Ltd. Received 28 November 1967. This research was supported in part by grant no. GM13554 from the U.S. Public Health Service. | |||||||||
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| Issue or Number: | 1 | |||||||||
| DOI: | 10.1016/0022-2836(68)90231-3 | |||||||||
| Record Number: | CaltechAUTHORS:20160601-093439043 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160601-093439043 | |||||||||
| Official Citation: | Rolf Knippers, Robert L. Sinsheimer, Process of infection with bacteriophage φX174, Journal of Molecular Biology, Volume 34, Issue 1, 1968, Pages 17-29, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(68)90231-3. (http://www.sciencedirect.com/science/article/pii/0022283668902313) | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 67529 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Ruth Sustaita | |||||||||
| Deposited On: | 01 Jun 2016 20:24 | |||||||||
| Last Modified: | 11 Nov 2021 03:50 |
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