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Vegetative lambda DNA:IV. Fractionation of Replicating Lambda DNA on Benzoylated-naphthoylated DEAE Cellulose

Kiger, John A., Jr. and Sinsheimer, Robert L. (1969) Vegetative lambda DNA:IV. Fractionation of Replicating Lambda DNA on Benzoylated-naphthoylated DEAE Cellulose. Journal of Molecular Biology, 40 (3). pp. 467-490. ISSN 0022-2836. https://resolver.caltech.edu/CaltechAUTHORS:20160601-093439344

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Abstract

Fractionation on a column of benzoylated-naphthoylated DEAE cellulose has been used successfully to separate pulse-labeled, replicating bacteriophage lambda DNA from completed phage DNA or completed ring DNA molecules. Sedimentation analysis of the heterogeneous replicating DNA in neutral and alkaline gradients indicates the presence of closed single-strand rings and of linear DNA strands ranging in length from smaller than to not quite twice that of the viral DNA strands. A model of DNA replication is presented which can, by the timely interaction of specific nuclease or polynucleotide ligase enzymes, account for the various DNA forms encountered.


Item Type:Article
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http://dx.doi.org/10.1016/0022-2836(69)90166-1DOIArticle
http://www.sciencedirect.com/science/article/pii/0022283669901661PublisherArticle
Additional Information:© 1969 Elsevier Ltd. Received 14 September 1968, Revised 13 December 1968. We thank Miss Patricia Ilg for competent assistance during the early stages of this work and for performing the experiment recorded in Fig. 2. We acknowledge many long and useful discussions with J. W. Sedat and J. S. Parkinson. This research has been supported in part by grant GM 13554 from the U.S. Public Health Service.
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Funding AgencyGrant Number
U.S. Public Health Service (USPHS)GM 13554
Issue or Number:3
Record Number:CaltechAUTHORS:20160601-093439344
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160601-093439344
Official Citation:John A. Kiger, Robert L. Sinsheimer, Vegetative lambda DNA, Journal of Molecular Biology, Volume 40, Issue 3, 1969, Pages 467-490, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(69)90166-1. (http://www.sciencedirect.com/science/article/pii/0022283669901661)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67530
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:01 Jun 2016 20:04
Last Modified:03 Oct 2019 10:06

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