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The Replication of Bacteriophage MS2: V. Proteins Specifically Associated with Infection

Haywood, Anne M. and Sinsheimer, Robert L. (1965) The Replication of Bacteriophage MS2: V. Proteins Specifically Associated with Infection. Journal of Molecular Biology, 14 (2). pp. 305-326. ISSN 0022-2836. doi:10.1016/S0022-2836(65)80184-X.

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Uninfected and MS2-infected Escherichia coli spheroplasts, treated with 10 μg/ml. of actinomycin D, were labeled, one with a [^(14)C]amino acid and the other with the corresponding [^3H]amino acid. The resultant lysates were combined, fractionated and the fractions differentially counted. Several protein fractions contained an increased ratio of counts from the infected culture to counts from the uninfected culture. These were: (1) a histidine-labeled fraction with an S value of about 41 s derived from a 13,000 g sediment; (2) a fraction sedimenting at 60 to 80 s and a fraction sedimenting at greater than 81 s, both of which were derived from the 105,000 g sediment (these fractions were observed with leucine or lysine labeling, but not with histidine labeling); (3) a fraction sedimenting at about 6 s from a 105,000 g sediment, labeled with histidine; (4) fractions from the 105,000 g supernatant which were eluted from a DEAE-Sephadex column at 0·22 M-NaCl and 0·26 M-NaCl in pH 7·6 buffer. Also, a fraction was isolated from both infected and uninfected cells, the synthesis of which seemed independent of the presence of actinomycin.

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Additional Information:© 1965 Elsevier Ltd. Received 7 June 1965. This research was supported, in part, by grant RG-6965 from t he U.S. Public Health Service.
Funding AgencyGrant Number
U.S. Public Health ServiceRG-6965
Issue or Number:2
Record Number:CaltechAUTHORS:20160601-112714418
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Official Citation:Anne M. Haywood, Robert L. Sinsheimer, The replication of bacteriophage MS2, Journal of Molecular Biology, Volume 14, Issue 2, 1965, Pages 305-326, ISSN 0022-2836, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67543
Deposited On:01 Jun 2016 19:34
Last Modified:11 Nov 2021 03:50

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