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A Biophysical Approach Towards Understanding the Transport Mechanism of an ABC L-Methionine Importer

Li, Qi W. (2016) A Biophysical Approach Towards Understanding the Transport Mechanism of an ABC L-Methionine Importer. Biophysical Journal, 110 (3). 139A-140A. ISSN 0006-3495. doi:10.1016/j.bpj.2015.11.794. https://resolver.caltech.edu/CaltechAUTHORS:20160603-160132094

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Abstract

ABC transporters are prevalent in many kingdoms of life. In E. coli, one third of the transporters are ABC transporters. Their proper functions are vital to cell survival. Multiple drug resistance (MDR) transporters are a type of ABC transporters. They pump the drug out of the cell before the drug can exert its effects. Multidrug resistance is a major challenge in cancer and infectious diseases. Such resistance interferes with treatments and leads to longer recovery times and higher mortality rates. In addition, ABC transporters are important in chloride conductance, cholesterol transport, and surface-antigen presentation. ABC transporters in general also play a role in the regulation of movements of small molecules, ions, and macromolecules across membranes. MetNI was discovered by Kadner in the 1970s to efficiently uptake L-methionine in E. coli. In the MetNI system, the interplay between transporter and its binding protein, MetQ delivers L-methionine to the cell. Using a biophysical method, SPR, the kinetics of MetQ binding MetNI have been characterized and rate constants are extrapolated. Using a thermophoresis technique, MST, dissociation constants of this protein-protein interaction with different mutations, substrates, and nucleotide have been quantified. A transport mechanism, which consists of rate and equilibrium constants is being developed. By synthesizing the knowledge on the ATP hydrolysis, binding, transport, and conformational change, we will gain a better understanding of mechanisms of this intricate and important process. Importantly, this model will be able to help predict activities and stability of other members of the type I ABC transporters.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.bpj.2015.11.794DOIArticle
http://www.sciencedirect.com/science/article/pii/S0006349515019773PublisherArticle
Additional Information:© 2016 Biophysical Society. Published by Elsevier Inc.
Issue or Number:3
DOI:10.1016/j.bpj.2015.11.794
Record Number:CaltechAUTHORS:20160603-160132094
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160603-160132094
Official Citation:Qi W. Li, A Biophysical Approach Towards Understanding the Transport Mechanism of an ABC L-Methionine Importer, Biophysical Journal, Volume 110, Issue 3, Supplement 1, 16 February 2016, Pages 139a-140a, ISSN 0006-3495, http://dx.doi.org/10.1016/j.bpj.2015.11.794. (http://www.sciencedirect.com/science/article/pii/S0006349515019773)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67666
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:04 Jun 2016 17:32
Last Modified:11 Nov 2021 03:52

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