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DNA-Mediated Redox Signaling in Bacterial Nucleotide Excision Repair by UvrC

Silva, Rebekah M. B. and Zhou, Andy and Grodick, Michael A. and Barton, Jacqueline K. (2016) DNA-Mediated Redox Signaling in Bacterial Nucleotide Excision Repair by UvrC. Biophysical Journal, 110 (3). 62A-63A. ISSN 0006-3495. http://resolver.caltech.edu/CaltechAUTHORS:20160606-074814901

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Abstract

Our laboratory has proposed redox signaling among DNA repair proteins containing 4Fe-4S clusters through DNA charge transport (DNA CT) as a first step in lesion detection. Recently, we have explored whether UvrC, the endonuclease in Nucleotide Excision Repair (NER), may also contain a 4Fe-4S cluster. A new purification route was developed to express an MBP-UvrC fusion protein, and evidence of a 4Fe-4S cluster was seen by UV-Visible (UV-Vis) and Electron Paramagnetic Resonance (EPR) spectroscopy. Like other 4Fe-4S repair proteins studied in our lab, MBP-UvrC can participate in DNA CT chemistry, as evidenced by its electrochemical activity on DNA-modified gold electrodes. Complementing in vivo genetic assays have been developed and indicate that DNA-mediated signaling between UvrC and other DNA-processing enzymes containing 4Fe-4S clusters is occurring. Taken together, these results have suggested that UvrC is part of a network of 4Fe-4S proteins that communicate using DNA CT to find lesions and maintain genomic integrity. Additional in vitro and in vivo characterization is underway to understand further the biological implications of the newly-discovered, DNA-mediated redox chemistry of UvrC.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.bpj.2015.11.403DOIArticle
http://www.sciencedirect.com/science/article/pii/S0006349515015866PublisherArticle
ORCID:
AuthorORCID
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2016 Biophysical Society. Published by Elsevier Inc.
Record Number:CaltechAUTHORS:20160606-074814901
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160606-074814901
Official Citation:Rebekah M.B. Silva, Andy Zhou, Michael A. Grodick, Jacqueline K. Barton, DNA-Mediated Redox Signaling in Bacterial Nucleotide Excision Repair by UvrC, Biophysical Journal, Volume 110, Issue 3, Supplement 1, 16 February 2016, Pages 62a-63a, ISSN 0006-3495, http://dx.doi.org/10.1016/j.bpj.2015.11.403. (http://www.sciencedirect.com/science/article/pii/S0006349515015866)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67668
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:06 Jun 2016 16:42
Last Modified:06 Jun 2016 16:42

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