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DNA-Mediated Redox Signaling in Bacterial Nucleotide Excision Repair by UvrC

Silva, Rebekah M. B. and Zhou, Andy and Grodick, Michael A. and Barton, Jacqueline K. (2016) DNA-Mediated Redox Signaling in Bacterial Nucleotide Excision Repair by UvrC. Biophysical Journal, 110 (3). 62A-63A. ISSN 0006-3495. doi:10.1016/j.bpj.2015.11.403.

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Our laboratory has proposed redox signaling among DNA repair proteins containing 4Fe-4S clusters through DNA charge transport (DNA CT) as a first step in lesion detection. Recently, we have explored whether UvrC, the endonuclease in Nucleotide Excision Repair (NER), may also contain a 4Fe-4S cluster. A new purification route was developed to express an MBP-UvrC fusion protein, and evidence of a 4Fe-4S cluster was seen by UV-Visible (UV-Vis) and Electron Paramagnetic Resonance (EPR) spectroscopy. Like other 4Fe-4S repair proteins studied in our lab, MBP-UvrC can participate in DNA CT chemistry, as evidenced by its electrochemical activity on DNA-modified gold electrodes. Complementing in vivo genetic assays have been developed and indicate that DNA-mediated signaling between UvrC and other DNA-processing enzymes containing 4Fe-4S clusters is occurring. Taken together, these results have suggested that UvrC is part of a network of 4Fe-4S proteins that communicate using DNA CT to find lesions and maintain genomic integrity. Additional in vitro and in vivo characterization is underway to understand further the biological implications of the newly-discovered, DNA-mediated redox chemistry of UvrC.

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Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2016 Biophysical Society. Published by Elsevier Inc.
Issue or Number:3
Record Number:CaltechAUTHORS:20160606-074814901
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Official Citation:Rebekah M.B. Silva, Andy Zhou, Michael A. Grodick, Jacqueline K. Barton, DNA-Mediated Redox Signaling in Bacterial Nucleotide Excision Repair by UvrC, Biophysical Journal, Volume 110, Issue 3, Supplement 1, 16 February 2016, Pages 62a-63a, ISSN 0006-3495, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67668
Deposited By: Tony Diaz
Deposited On:06 Jun 2016 16:42
Last Modified:11 Nov 2021 03:52

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