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Dynamics of Epigenetic Regulation at the Single-Cell Level

Bintu, Lacramioara (2016) Dynamics of Epigenetic Regulation at the Single-Cell Level. Biophysical Journal, 110 (3). 317A-318A. ISSN 0006-3495. https://resolver.caltech.edu/CaltechAUTHORS:20160606-083428365

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Abstract

Chromatin regulation enables cells to establish and remember gene expression states. However, how chromatin regulators control gene expression in single cells, quantitatively and over time, has remained unclear. Using time-lapse microscopy, we addressed this question for four biochemically diverse chromatin regulators (DNMT3B, HDAC4, EED, KRAB). For all four factors, silencing and reactivation occurred in all-or-none events, allowing these regulators to modulate the fraction of silenced cells over time, rather than the level of gene expression. These dynamics could be described by a simple 3-state model involving stochastic transitions between active, reversibly silent, and irreversibly silent states. The specific transition rates of each regulator produce distinct timescales of silencing (hours to days), and types of epigenetic memory (transient—HDAC4, permanent—DNMT3B, and a hybrid of the two—EED and KRAB). These results provide a framework for understanding and engineering mammalian chromatin regulation.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.bpj.2015.11.1704DOIArticle
http://www.sciencedirect.com/science/article/pii/S0006349515028878PublisherArticle
Additional Information:© 2016 Biophysical Society. Published by Elsevier Inc.
Issue or Number:3
Record Number:CaltechAUTHORS:20160606-083428365
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160606-083428365
Official Citation:Lacramioara Bintu, Dynamics of Epigenetic Regulation at the Single-Cell Level, Biophysical Journal, Volume 110, Issue 3, Supplement 1, 16 February 2016, Pages 317a-318a, ISSN 0006-3495, http://dx.doi.org/10.1016/j.bpj.2015.11.1704. (http://www.sciencedirect.com/science/article/pii/S0006349515028878)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:67677
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:06 Jun 2016 16:33
Last Modified:03 Oct 2019 10:07

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